Sex-Related Differences in Anxiety and Functional Recovery after Spinal Cord Injury in Mice
Sex-Related Differences in Anxiety and Functional Recovery after Spinal Cord Injury in Mice
复制标题
DOI：
10.1089/neu.2019.6929
复制
发表时间：
2020-07-30
影响因子：
4.2
通讯作者：
Ishibashi, Yasuyuki
中科院分区：
文献类型：
--
作者：
Fukutoku, Tatsuhiro;Kumagai, Gentaro;Ishibashi, Yasuyuki
据报道，雌性大鼠在脊髓损伤(SCI)后的功能恢复和神经保护方面具有性别相关的优势。然而，雌性和雄性大鼠脊髓损伤后焦虑和神经功能之间的联系仍不清楚。这项研究的目的是检验成年C57/BL6雄性和雌性小鼠在脊髓损伤后焦虑和神经功能障碍方面的性别差异。在第10个胸椎节段椎板切除后，造成脊髓挫伤。假手术组仅行T10椎板切除术。脊髓损伤后6周进行行为测试(焦虑、运动/感觉功能)。对震中的脊髓和保留的有髓区域进行组织学评估。使用Spearman相关系数分析焦虑与运动/感觉功能或组织学参数之间的相关性。雌性和雄性小鼠在脊髓损伤后表现出明显高于损伤前的焦虑样行为。在脊髓损伤后，雌性小鼠的焦虑感明显高于雄性小鼠。两组患者的运动/感觉功能和组织学特征无明显差异。雌性小鼠SCI后2周焦虑样行为与感觉功能显著相关，雄性小鼠SCI后2、4、6周焦虑样行为与运动功能显著相关。在雌性和雄性小鼠中，焦虑样行为与震中脊髓面积没有显著相关性。我们的结果显示，雌性小鼠在脊髓损伤后变得比雄性小鼠更焦虑。脊髓损伤后的焦虑样行为可能与功能恢复有关，改善焦虑状态可能影响损伤后的功能恢复。
It has been reported that female rats have a sex-related advantage in functional recovery and neuroprotection after spinal cord injury (SCI). However, the association between anxiety and neurological function after SCI in female and male rats remains unclear. The aim of this study was to examine sex-related differences in anxiety and neurological dysfunction after SCI in adult C57/BL6 male and female mice. After laminectomy at the 10th thoracic level, a contusive SCI was induced. The sham group received only a T10 laminectomy. Behavior testing (anxiety, motor/sensory function) was performed for 6 weeks after SCI. The spinal cord and preserved myelinated areas at the epicenter were histologically evaluated. Correlations between anxiety and motor/sensory function or histological parameters were analyzed using the Spearman correlation coefficient. Female and male mice showed significantly higher anxiety-like behaviors after SCI than before SCI. Anxiousness was significantly higher in female mice than in male mice after SCI. There was no significant difference in motor/sensory functions and histological features between the two groups. Anxiety-like behaviors were significantly correlated with sensory function at 2 weeks after SCI in female mice and with motor function at 2, 4, and 6 weeks after SCI in male mice. Anxiety-like behaviors were not significantly correlated with the spinal cord area at the epicenter in female and male mice. Our results revealed that female mice became more anxious than male mice after SCI. Anxiety-like behavior after SCI may be associated with functional recovery, and improving anxiety may affect functional recovery after injury.