Individual Neuronal Subtypes Exhibit Diversity in CNS Myelination Mediated by Synaptic Vesicle Release.
Individual Neuronal Subtypes Exhibit Diversity in CNS Myelination Mediated by Synaptic Vesicle Release.
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DOI：
10.1016/j.cub.2016.03.070
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发表时间：
2016-06-06
期刊：
影响因子：
--
通讯作者：
Lyons DA
中科院分区：
文献类型：
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作者：
Koudelka S;Voas MG;Almeida RG;Baraban M;Soetaert J;Meyer MP;Talbot WS;Lyons DA
Regulation of myelination by oligodendrocytes in the CNS has important consequences for higher-order nervous system function (e.g.,), and there is growing consensus that neuronal activity regulates CNS myelination (e.g.,) through local axon-oligodendrocyte synaptic-vesicle-release-mediated signaling. Recent analyses have indicated that myelination along axons of distinct neuronal subtypes can differ, but it is not known whether regulation of myelination by activity is common to all neuronal subtypes or only some. This limits insight into how specific neurons regulate their own conduction. Here, we use a novel fluorescent fusion protein reporter to study myelination along the axons of distinct neuronal subtypes over time in zebrafish. We find that the axons of reticulospinal and commissural primary ascending (CoPA) neurons are among the first myelinated in the zebrafish CNS. To investigate how activity regulates myelination by different neuronal subtypes, we express tetanus toxin (TeNT) in individual reticulospinal or CoPA neurons to prevent synaptic vesicle release. We find that the axons of individual tetanus toxin expressing reticulospinal neurons have fewer myelin sheaths than controls and that their myelin sheaths are 50% shorter than controls. In stark contrast, myelination along tetanus-toxin-expressing CoPA neuron axons is entirely normal. These results indicate that while some neuronal subtypes modulate myelination by synaptic vesicle release to a striking degree in vivo, others do not. These data have implications for our understanding of how different neurons regulate myelination and thus their own function within specific neuronal circuits. Live imaging of myelin sheath dynamics along single axons over time in zebrafish Vesicular release from reticulospinal axons required for normal myelination Vesicular release from CoPA axons not required for myelination Evidence of neuronal subtype diversity of activity-regulated myelination Neuronal activity can regulate myelination, which in turn affects nervous system function. Koudelka et al. use zebrafish to image myelination over time along individual axons in vivo. They find that while some neuronal subtypes regulate myelination by vesicular release, others do not, indicating striking diversity in activity-regulated myelination.
