Speciation of trace elements in human serum by micro anion exchange chromatography coupled with inductively coupled plasma mass spectrometry.
Speciation of trace elements in human serum by micro anion exchange chromatography coupled with inductively coupled plasma mass spectrometry.
复制标题
通过微阴离子交换色谱法结合电感耦合等离子体质谱法对人血清中的微量元素形态进行分析。
DOI：
10.1016/j.ab.2011.11.004
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发表时间：
2012
影响因子：
2.9
通讯作者：
E. Mocchegiani
中科院分区：
文献类型：
--
作者：
M. Malavolta;F. Piacenza;A. Basso;R. Giacconi;L. Costarelli;S. Pierpaoli;E. Mocchegiani
人类血清中必需微量元素的形态分析为研究人体营养状况和调节转运过程、急性期反应和抗氧化损伤的稳态机制提供了重要信息。阴离子交换高效液相色谱（HPLC）与电感耦合等离子体质谱（ICP-MS）相结合已被证明是一种有用的物种形成工具。在这里，我们描述了一种快速的方法，可以应用于在1ml的血清中进行铁，铜，锌和硒的形态分析。该方法采用安装在串联高效液相色谱系统上的整体阴离子交换微柱与ICP-MS检测器在线耦合。色谱分离与先前报道的相似，但在时间和样品要求方面有相当大的增益。大多数物种的可重复性是可以接受的。使用我们的方法，我们能够发现不同市售微量元素参考物质之间的物种特异性差异。由于所选择的采集血液的方法可能会干扰物种的形成，因此采用该方法比较了来自成年健康志愿者的肝素化血浆、乙二胺四乙酸（EDTA）血浆和血清。正如预期的那样，EDTA强烈影响形态分析（特别是铁和锌），而使用锂-肝素（Li-He）作为抗凝剂所引起的变化似乎最小。
Speciation analysis of essential trace elements in human serum provides important information on nutritional status and homeostatic mechanisms regulating transport processes, acute phase reactions, and protection against oxidative damage. Anion exchange high-performance liquid chromatography (HPLC) combined with inductively coupled plasma mass spectrometry (ICP–MS) has proved to be a useful tool in speciation. Here we describe a fast method that can be applied to carry out the speciation of Fe, Cu, Zn, and Se in as little as 1ml of serum. The method employs monolithic anion exchange micro columns installed on a tandem HPLC system coupled on-line with an ICP–MS detector. The chromatographic separation is similar to those reported previously but with considerable gain in terms of time and sample requirement. Reproducibility is acceptable for most species. Using our method, we were able to find species-specific differences between different commercially available trace element reference materials. Because the method chosen to collect blood might interfere with speciation, the proposed methodology was used to compare heparinized plasma, ethylenediaminetetraacetic acid (EDTA) plasma, and serum from adult healthy volunteers. As expected, EDTA strongly affects speciation analysis (especially for Fe and Zn), whereas changes due to the use of lithium–heparin (Li–He) as anticoagulant appear to be minimized.