Hydrogen ion diffusion in dog gastric mucus glycoprotein: effect of associated lipids and covalently bound fatty acids.
Hydrogen ion diffusion in dog gastric mucus glycoprotein: effect of associated lipids and covalently bound fatty acids.
复制标题
狗胃粘液糖蛋白中的氢离子扩散：相关脂质和共价结合脂肪酸的影响。
DOI：
10.1016/0006-291x(84)91334-2
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发表时间：
1984
影响因子：
3.1
通讯作者：
Slomiany,BL
中科院分区：
文献类型：
--
作者：
Sarosiek,J;Slomiany,A;Takagi,A;Slomiany,BL
研究了狗胃粘液糖蛋白结合的中性脂、糖脂和磷脂以及共价结合脂肪酸对糖蛋白抑制氢离子扩散能力的影响。将天然形式的纯化粘液糖蛋白放置在一个特殊设计的两室室中的等摩尔(0.155M)盐酸和氯化钠溶液之间，与一层氯化钠相比，对氢离子的渗透性降低了90%。相关脂类的提取导致糖蛋白对氢离子的渗透性增加68%，而去除共价结合的脂肪酸则进一步增加了6%的扩散速度。脱脂糖蛋白与其中性脂的再结合使其对氢离子的通透性降低了34%，糖脂降低了11%，磷脂降低了23%。由于中性脂占糖蛋白脂的47%，糖脂占41.1%，磷脂占11.9%，因此脱脂糖蛋白与磷脂重新结合后的通透性定量下降是中性脂的2.7倍，是糖脂的7.3倍。
The effect of neutral lipids, glycolipids and phospholipids associated with dog gastric mucus glycoprotein, and that of covalently bound fatty acids on the ability of glycoprotein to retard the diffusion of hydrogen ion was investigated. Purified mucus glycoprotein in its native form, placed between equimolar (0.155M) solutions of HCl and NaCl in a specially designed two-compartment chamber, caused a 90% reduction in permeability to hydrogen ion when compared with a layer of NaCl. Extraction of associated lipids lead to a 68% increase in permeability of the glycoprotein to hydrogen ion, while removal of the covalently bound fatty acids increased further the diffusion rate by 6%. Reassociation of the delipidated glycoprotein with its neutral lipids reduced the permeability to hydrogen ion by 34%, an 11% reduction was obtained with glycolipids, and 23% with phospholipids. Since neutral lipids account for 47% of the glycoprotein lipids, glycolipids 41.1% and phospholipids 11.9%, the quantitative decrease in permeability of the delipidated glycoprotein following its reassociation with phospholipids is 2.7 times greater than that of neutral lipids and 7.3 times greater than that of glycolipids.