Biofilms in the edentulous oral cavity.
Biofilms in the edentulous oral cavity.
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DOI：
10.1111/j.1532-849x.2008.00301.x
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发表时间：
2008-07-01
期刊：
影响因子：
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通讯作者：
Socransky, Sigmund S
中科院分区：
文献类型：
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作者：
Sachdeo, Amit;Haffajee, Anne D;Socransky, Sigmund S
目的：口腔为微生物定植提供了多种表面。这些表面产生不同复杂程度的生物膜，每个个体都是独一无二的。有几项研究观察了齿状患者的生物膜。关于义齿或无牙患者软组织上的生物膜的研究一直很有限。本研究的目的是提供描述无牙颌患者口腔微生物生态关系的有意义的数据，并评价无牙颌患者戴全口义齿时硬、软组织表面和唾液中的微生物区系。材料和方法：选择61名上下颌全口义齿患者。从每个受试者的28颗义齿上取“牙周上”生物膜样本。生物膜样本也取自舌头的背部、侧面和腹面、口底、颊粘膜、硬腭、前庭/嘴唇、附着性牙龈和唾液。采用棋盘DNA-DNA杂交法分别对41种细菌的含量进行分析。结果：牙周病原菌如伴生放线杆菌、牙龈卟啉单胞菌等牙周病原菌在无牙合受试者的牙周标本中明显存在。软组织表面样本中的微生物图谱因场地位置不同而不同。来自舌背的样本细菌数最高，其次是附着性牙龈和舌侧表面，而口腔粘膜和唇前庭样本的平均细菌数最低。通过对供试物种比例的聚类分析，形成了三个聚类群。第一簇包括唾液、牙龈上菌斑、舌侧和舌背表面。第二组包括其他六个软组织表面。结论：本研究的主要发现之一是在无牙颌受试者中检测到牙周病原体：伴生放线菌和牙龈假单胞菌，因为这些物种被认为在移除所有天然牙后消失。这一发现对未来的牙科治疗和个人的总体健康有一定的影响。在不同的软组织表面可以看到不同的微生物定植模式。因此，这项调查为确定与无牙患者软面(粘膜)和硬面(义齿)相关的微生物提供了第一步。这项研究还提供了有意义的数据，描述了无牙颌受试者口腔中的微生物生态关系。作者认为，这项研究是第一次对全口义齿佩戴对象的微生物区系进行全面评估。
PURPOSE: The oral cavity presents numerous surfaces for microbial colonization. These surfaces produce biofilms of differing complexities unique to each individual. Several studies have looked at biofilms in dentate patients. There has been limited research regarding biofilms on dentures or soft tissues of edentulous patients. The purpose of the present investigation was to provide meaningful data describing microbial ecological relationships in the oral cavity of edentulous patients and to evaluate the microbiota on hard and soft tissue surfaces and saliva in edentulous patients wearing complete dentures.MATERIALS AND METHODS: Sixty-one edentulous subjects with complete maxillary and mandibular dentures were recruited. "Supragingival" biofilm samples were taken from 28 denture teeth for each subject. Biofilm samples were also taken from the dorsal, lateral, and ventral surfaces of the tongue, floor of mouth, buccal mucosa, hard palate, vestibule/lip, "attached gingiva," and saliva. Samples were individually analyzed for their content of 41 bacterial species using checkerboard DNA-DNA hybridization. Levels and proportions of each species were determined for every sample location.RESULTS: Periodontal pathogens such as Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis were clearly present in the samples from the edentulous subjects. Microbial profiles in samples from the soft tissue surfaces differed among site locations. Samples from the dorsum of the tongue exhibited the highest bacterial counts followed by the "attached gingiva" and the lateral surfaces of the tongue, while the lowest mean counts were found in samples from the buccal mucosa and labial vestibules. Using cluster analysis of the proportions of the test species, three clusters were formed. The first cluster comprised saliva, supragingival plaque, and the lateral and dorsal surfaces of the tongue. The second cluster comprised the other six soft tissue surfaces. Species on the denture palate formed a third cluster.CONCLUSIONS: One of the major findings in this study was the detection of periodontal pathogens, A. actinomycetemcomitans and P. gingivalis, in the edentulous subjects, as these species were thought to disappear after removal of all natural teeth. This finding has implications regarding future dental treatment and the general health of individuals. Distinct patterns of microbial colonization were seen on the different soft tissue surfaces. Thus, this investigation provided the first step in defining the organisms that are associated with edentulous patients on both soft (mucosa) and hard surfaces (denture). The study also provided meaningful data that described microbial ecological relationships in the oral cavity of edentulous subjects. The authors believe that this study is the first comprehensive assessment of the microbiota in the complete denture-wearing subject.