Anti‐herbicide single‐chain antibody expression confers herbicide tolerance in transgenic plants
Anti‐herbicide single‐chain antibody expression confers herbicide tolerance in transgenic plants
复制标题
抗除草剂单链抗体表达赋予转基因植物除草剂耐受性
DOI：
10.1016/s0014-5793(03)00871-8
复制
发表时间：
2003
期刊：
影响因子：
3.5
通讯作者：
I. Yamaguchi
中科院分区：
文献类型：
--
作者：
J. Eto;Yoshihito Suzuki;H. Ohkawa;I. Yamaguchi
将抗氯苯胺灵单链可变片段 (scFv) 基因引入拟南芥中，以在四个不同的亚细胞区室中的每一个中表达抗体片段。通过靶向内质网或质外体空间中的片段，或通过将片段表达为糖基磷脂酰肌醇锚定蛋白来检测转基因植物中scFv的积累，而通过靶向胞质中的片段则不能检测到积累。与非转化体相比，在内质网中高水平积累 scFv 基因的转基因植物对氯苯胺灵的耐受性增强。
An anti‐chlorpropham single‐chain variable‐fragment (scFv) gene was introduced intoArabidopsisin a manner to express the antibody fragment in each of four different subcellular compartments. The accumulation of scFv in transgenic plants was detected by targeting the fragment in the endoplasmic reticulum or apoplastic space, or by expressing the fragment as a glycosylphosphatidylinositol‐anchored protein, while no accumulation could be detected by targeting the fragment in the cytosol. Transgenic plants accumulating the scFv gene at a high level in the endoplasmic reticulum had enhanced tolerance to chlorpropham in comparison with the non‐transformants.