Label-free biochemical quantitative phase imaging with mid-infrared photothermal effect
Label-free biochemical quantitative phase imaging with mid-infrared photothermal effect
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DOI：
10.1364/optica.390186
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发表时间：
2020-04-20
期刊：
影响因子：
10.4
通讯作者：
Ideguchi, Takuro
中科院分区：
文献类型：
--
作者：
Tamamitsu, Miu;Toda, Keiichiro;Ideguchi, Takuro
无标签光学成像因其无损特性在生物学和医学中具有重要的应用价值。定量相位成像（QPI）和分子振动成像（MVI）是两种最成功的无标记方法，分别提供形态和生化信息。在过去的几十年里，随着这些技术的成熟，它们已经实现了许多应用；然而，由于它们依赖于不同的光-物质相互作用，它们的无标签对比本质上是互补的，难以整合。在这里，我们提出了一种统一的成像方案，利用中红外光热效应同时和原位获取QPI框架中单个细胞的定量相位和分子振动对比。亚细胞形态学和生化无标记测量的强大整合可能会带来新的分析，特别是对于研究复杂和脆弱的生物现象，如药物输送，细胞疾病和干细胞发育，在低光毒性下需要长时间观察未受干扰的细胞。(C) 2020年美国光学学会根据OSA开放获取出版协议的条款
Label-free optical imaging is valuable in biology and medicine because of its non-destructive nature. Quantitative phase imaging (QPI) and molecular vibrational imaging (MVI) are the two most successful label-free methods, providing morphological and biochemical information, respectively. These techniques have enabled numerous applications as they have matured over the past few decades; however, their label-free contrasts are inherently complementary and difficult to integrate due to their reliance on different light-matter interactions. Here we present a unified imaging scheme with simultaneous and in situ acquisition of quantitative phase and molecular vibrational contrasts of single cells in the QPI framework using the mid-infrared photothermal effect. The robust integration of subcellular morphological and biochemical label-free measurements may enable new analyses, especially for studying complex and fragile biological phenomena such as drug delivery, cellular disease, and stem cell development, where long-time observation of unperturbed cells is needed under low phototoxicity. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement