Temporal and spatial incidence of alleles conferring knockdown resistance to pyrethroids in the peach-potato aphid, Myzus persicae (Hemiptera: Aphididae), and their association with other insecticide resistance mechanisms
Temporal and spatial incidence of alleles conferring knockdown resistance to pyrethroids in the peach-potato aphid, Myzus persicae (Hemiptera: Aphididae), and their association with other insecticide resistance mechanisms
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DOI：
10.1017/s0007485307004889
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发表时间：
2007-06-01
影响因子：
1.9
通讯作者：
Denholm, I.
中科院分区：
文献类型：
--
作者：
Anstead, J. A.;Mallet, J.;Denholm, I.
桃马铃薯蚜虫 Myzus persicae (sulzer) 是世界各地重要的耕地害虫物种。杀虫剂的广泛使用导致了对大多数化学类别的抗性选择，包括有机氯、有机磷酸酯、氨基甲酸酯和拟除虫菊酯。对拟除虫菊酯的抗性通常是对位型钠通道蛋白突变（击倒抗性或 kdr）的结果。在桃桃中，敲低抗性与两种氨基酸取代相关：L1014F (kdr) 和 M918T (super-kdr)。在这项研究中，使用等位基因区分聚合酶链反应测定诊断这些突变的时间和空间分布，并与其他耐药机制（修饰的乙酰胆碱酯酶（MACE）和升高的羧酸酯酶）一起进行了研究。样本是从英国、欧洲大陆、津巴布韦和澳大利亚东南部采集的。 kdr 突变和羧酸酯酶升高广泛分布，几乎在每个国家都有记录。 MACE 和 super-kdr 在欧洲广泛存在，但在澳大利亚样本中不存在。在整个过程中检测到 kdr 和 super-kdr 的杂合子过量意味着对这些抗性突变纯合个体的强烈选择。在英国发现的分布模式似乎表明，在没有杀虫剂压力的情况下，任何基因型中对超级 kdr（但不是 kdr）突变的强烈选择。不同的抗性机制之间存在显着的关联（连锁不平衡），这可能是由于孤雌生殖导致重组缺乏而促进的。
The peach-potato aphid, Myzus persicae (sulzer), is an important arable pest species throughout the world. Extensive use of insecticides has led to the selection of resistance to most chemical classes including organochlorines, organophosphates, carbamates and pyrethroids. Resistance to pyrethroids is often the result of mutations in the para-type sodium channel protein (knockdown resistance or kdr). In M. persicae, knockdown resistance is associated with two amino-acid substitutions, L1014F (kdr) and M918T (super-kdr). In this study, the temporal and spatial distributions of these mutations, diagnosed using an allelic discriminating polymerase chain reaction assay, were investigated alongside other resistance mechanisms (modified acetylcholinesterase (MACE) and elevated carboxylesterases). Samples were collected from the UK, mainland Europe, Zimbabwe and south-eastern Australia. The kdr mutation and elevated carboxylesterases were widely distributed and recorded from nearly every country. MACE and super-kdr were widespread in Europe but absent from Australian samples. The detection of a strongly significant heterozygote excess for both kdr and super-kdr throughout implies strong selection against individuals homozygous for these resistance mutations. The pattern of distribution found in the UK seemed to indicate strong selection against the super-kdr (but not the kdr) mutation in any genotype, in the absence of insecticide pressure. There was a significant association (linkage disequilibrium) between different resistance mechanisms, which was probably promoted by a lack of recombination due to parthenogenesis.