CD200 is a ligand for all members of the CD200R family of immunoregulatory molecules
CD200 is a ligand for all members of the CD200R family of immunoregulatory molecules
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DOI：
10.4049/jimmunol.172.12.7744
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发表时间：
2004-06-15
影响因子：
4.4
通讯作者：
Marsden, PA
中科院分区：
文献类型：
--
作者：
Gorczynski, R;Chen, ZQ;Marsden, PA
CD 200 Fc是一种包含CD 200胞外结构域和鼠IgG 2a Fc区的嵌合分子，在与髓系和T细胞谱系细胞上表达的细胞表面受体CD 200 R接合后调节免疫应答。最近的一份报告将注意力集中在CD 200 R家族上，但得出的结论是只有一个成员使用CD 200作为其配体。我们还克隆和测序了一个家族的CD 200受体，但确定了一个氨基末端的三个异构体不承认以前的研究人员。我们用FITC标记的CD 200 Fc通过流式细胞术显示，用所有CD 200 R亚型转染的COS 7细胞结合CD 200作为配体，尽管这种结合的功能结果在不同亚型之间可能不同。针对CD 200 R1/R4同种型的mAb以与CD 200 Fc相当的方式改变了IL-2/IL-4细胞因子的产生并抑制了CTL应答，在加入抗CD 200 R2/R3后观察到显著更小的作用。
CD200Fc, a chimeric molecule including the extracellular domain of CD200 and a murine IgG2a Fc region, regulates immune responses following engagement of a cell surface receptor, CD200R, expressed on cells of the myeloid and T cell lineage. A recent report focused attention on a family of CD200Rs, but concluded that only one member used CD200 as its ligand. We have also cloned and sequenced a family of CD200Rs, but identify an amino terminus to two of the three isoforms not recognized by previous researchers. We show by FACS, using FITC-labeled CD200Fc, that COS7 cells transfected with all CD200R isoforms bind CD200 as ligand, although the functional consequences of this binding likely differs between the different isoforms. mAbs directed against the CD200 R1/R4 isoforms altered IL-2/IL-4 cytokine production and suppressed CTL responses in a fashion comparable to CD200Fc, with a significantly lesser effect seen following addition of anti-CD200 R2/R3.