Comparison of Methods for the Reconstruction of the Hepatic Artery in Mouse Orthotopic Liver Transplantation.
Comparison of Methods for the Reconstruction of the Hepatic Artery in Mouse Orthotopic Liver Transplantation.
复制标题
小鼠原位肝移植肝动脉重建方法比较
DOI：
10.1371/journal.pone.0133030
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Liu Q
中科院分区：
文献类型：
--
作者：
Pan N;Liu Z;He J;Li S;Lv X;Wang L;Liu Q
研究背景动脉化原位肝移植（AOLT）小鼠模型在生物医学研究中具有重要作用。肝动脉重建的缝合吻合方法复杂，并发症和失败率高。因此，我们开发并评估了一种新的小鼠AOLT模型。材料与方法选用雄性近交系C57 BL/6小鼠。采用连续缝合法连接肝上下腔静脉（SHVC）。门静脉与肝下下腔静脉（IHVC）按“二袖套法”连接。胆总管通过胆道支架连接。采用支架（G3组）或主动脉干（G2组）重建肝动脉。一周后通过横切移植物附近的动脉来验证肝动脉的通畅性。术后1周观察受体存活率、血清丙氨酸氨基转移酶（ALT）水平、肝脏病理改变、细胞凋亡和坏死情况。结果G3组10只小鼠中有8只肝动脉通畅，G2组10只小鼠中有6只肝动脉通畅。3组间7天存活率、坏死和凋亡程度及TGF-β水平差异无统计学意义（P>0.05）。G3组ALT水平及手术时间均显著低于G2组和G1组（P均<0.05）。结论内支架重建肝动脉具有手术时间短、通畅率高的优点。该模型简化了肝动脉吻合，值得在生物医学研究领域推广。
Background The mouse model of arterialized orthotopic liver transplantation (AOLT) has played an important role in biomedical research. The available methods of sutured anastomosis for reconstruction of the hepatic artery are complicated, resulting in a high incidence of complications and failure. Therefore, we developed and evaluated a new model of AOLT in mice. Materials and methods Male inbred C57BL/6 mice were used in this study. A continuous suture approach was applied to connect the suprahepatic inferior vena cava (SHVC). The portal vein and infrahepatic inferior vena cava (IHVC) were connected according to the "two-cuff" method. The common bile duct was connected by a biliary stent. We used the stent (G3 group) or aortic trunk (G2 group) to reconstruct the hepatic artery. The patency of the hepatic artery was verified by transecting the artery near the graft after one week. The survival rate of the recipients and serum alanine aminotransferase (ALT) levels, hepatic pathologic alterations, apoptosis and necrosis were observed at one week postoperatively. Results The patency of the hepatic artery was verified in eight of ten mice in G3 and in six of ten mice in G2. The 7-day survival rate, extents of necrosis and apoptosis, and TGF-β levels were not significantly different among the three groups (P>0.05). However, the serum ALT levels and operation time were markedly lower in G3 compared with G2 or G1 (both P<0.05). Conclusions Reconstruction of the hepatic artery using a stent can be performed quickly with a high rate of patency. This model simplifies hepatic artery anastomosis and should be promoted in the field of biomedical research.