Early detection of HIV infection in a newborn.
Early detection of HIV infection in a newborn.
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新生儿艾滋病毒感染的早期检测。
DOI：
10.1056/nejm198701293160510
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
K. Rosenthal
中科院分区：
文献类型：
--
作者：
D. Harnish;O. Hammerberg;I. Walker;K. Rosenthal
先天性感染的诊断受到儿童免疫系统发育不良和母体抗体经胎盘通过的阻碍。作者描述了一种用于诊断新生儿HIV感染的原位杂交方法。用密度离心法从母亲和婴儿中分离单核细胞，用植物血凝素和白细胞介素-2培养2周，用35 S标记的HIV特异性cDNA探针检测淋巴细胞。一周后，3.4%的母体细胞和3.8%的婴儿细胞呈阳性。在2周结束时，<1%的母体细胞呈阳性，而3.9%的婴儿淋巴细胞仍呈阳性。这种方法是作为一种手段，提供了先天性艾滋病毒感染的早期诊断。吉莱斯皮
The diagnosis of congenital infections is hampered by the poor development of the child's immune system and transplacental passage of maternal antibody. The correspondents describe anin situhybridization method for the diagnosis of HIV infection in the newborn. Mononuclear cells were isolated from a mother and baby by density centrifugation and cultured with phytohaemagglutinin and interleukin-2 for 2 weeks.35S-labelled HIV-specific cDNA was used to probe the lymphocytes. After one week 3.4% of maternal cells, and 3.8% of the infant cells were positive. At the end of 2 weeks <1% of maternal cells were positive whereas 3.9% of the baby's lymphocytes were still positive. This method is presented as a means of providing an early diagnosis of congenital HIV infection.newline˜S.H. Gillespie
获得性免疫缺陷综合征的母婴传播。
影响因子：
8
作者：
Cowan,MJ;Hellmann,D;Chudwin,D;Wara,DW;Chang,RS;Ammann,AJ
通讯作者：
Ammann,AJ