Polysaccharide-coated beads platform for biomolecule analysis: Evolution of SiO2-based suspension arrays
Polysaccharide-coated beads platform for biomolecule analysis: Evolution of SiO2-based suspension arrays
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DOI：
10.1016/j.carbpol.2010.08.059
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发表时间：
2011-01
影响因子：
11.2
通讯作者：
Rong Cao;Demin Duan;Li Jiang;Zhuoxuan Lu;Fang Bao;Ke-xiao Zheng;Jiong Li
中科院分区：
文献类型：
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作者：
Rong Cao;Demin Duan;Li Jiang;Zhuoxuan Lu;Fang Bao;Ke-xiao Zheng;Jiong Li
图形编码的基于SiO2的悬浮阵列通常通过流行的脱氨基硅烷化学与2D基质一起制造，用于附着各种生物分子，包括肽、蛋白质、核酸探针等。鉴于相对较低的固定效率，我们开发了一种制备多糖包覆的二氧化硅珠的新方法，其可以增加生物分子在二氧化硅微球上的结合能力，提高生物分子在各种变性条件下的稳定性能。在这项研究中，两种代表性的多糖，壳聚糖和海藻酸钠（NaAlg），用于表面涂层。并研究了两种固定化蛋白质的方法。在优化的实验条件下，NaAlg包被的微球具有良好的生物分子结合能力，使荧光信号增加了近80%。出席所获得的结果，我们相信，所提出的方法可能具有显着的潜力，为新型的二氧化硅为基础的悬浮阵列技术。
Graphically encoded SiO2-based suspension arrays are often manufactured with the 2D matrixes by popular aldehyde–aminosilane chemistry for the attachment of a variety of biomolecules, including peptides, proteins, nucleic acid probes, etc. In view of the relatively low immobilization efficiency, we have developed a novel method to prepare polysaccharide-coated silica beads, which can increase the binding capacity of biomolecules on silica beads and improve the performance of the stability of biomolecules under various denaturing conditions. In this study, two kinds of representative polysaccharides, chitosan and sodium alginate (NaAlg), were used for the surface coating. And two procedures for the immobilization of protein on NaAlg-coated beads were also studied. Under the optimized experimental conditions, NaAlg-coated beads had excellent biomolecular binding capacities which increased the fluorescence signal by almost 80%. Attending to the results obtained, we believe that the proposed methods may have significant potential for novel SiO2-based suspension array technology.