Emergence of a new type of porcine circovirus in swine (PCV): A type 1 and type 2 PCV recombinant
Emergence of a new type of porcine circovirus in swine (PCV): A type 1 and type 2 PCV recombinant
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DOI：
10.1016/j.vetmic.2009.09.072
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发表时间：
2010-07-29
影响因子：
3.3
通讯作者：
Harel, Josee
中科院分区：
文献类型：
--
作者：
Gagnon, Carl A.;Music, Nedzad;Harel, Josee
2008年9月下旬，来自经历猪繁殖与呼吸综合征(PRRS)急性暴发的仔猪的组织样本被提交给蒙特利尔大学的兽医诊断服务。进行了多种诊断试验，包括多重实时定量聚合酶链式反应(MrtqPCR)检测和区分猪圆环病毒(PCV)2a和2b型在肺和淋巴结中的基因。用mrtqPCR检测猪样本PCV2a呈阳性，但得到了奇怪的结果。以PCV2的ORF1和ORF2为靶点的mrtqPCR探针的CT值与预期不符，提示PCV2病毒基因组中存在基因组变异。最终，共有3例mrtqPCR异常结果的诊断病例被调查。经过病毒分离和序列分析，在这三例病例中发现了一种新的PCV类型。根据序列分析，新的PCV基因组包含PCV1的ORF1和PCV2a的ORF2，其与PCV1、PCV2a和2b的全基因组核苷酸同源性分别为86.4%、88.7%和86.5%。考虑到Segales等人的命名法，建议命名这一新的PCV。(2008)，并首先指出ORF1的原产地，第二注明ORF2的原产地。因此，建议将这一新的PCV命名为PCV1/2a。在加拿大魁北克，PCV1/2a的流行率似乎非常低(PCV阳性病例的2.5%)，其来源目前仍有争议。(C)2010爱思唯尔B.V.保留所有权利。
In late September 2008, tissue samples from piglets experiencing an acute outbreak of porcine reproductive and respiratory syndrome (PRRS) were submitted to the Veterinary diagnostic service of the University of Montreal. Several diagnostic assays were performed including a multiplex real-time quantitative PCR assay (mrtqPCR) for the detection and differentiation of porcine circovirus (PCV) type 2a and 2b genotypes in the lung and lymph nodes. The pig samples were found to be positive for PCV2a using the mrtqPCR but odd results were obtained. The Ct values obtained with mrtqPCR probes targeting the ORF1 and ORF2 of PCV2 were not as expected which suggested the presence of genomic variations in the PCV2 viral genome. Ultimately, a total of three diagnostic cases with mrtqPCR unusual results were investigated. After virus isolation and sequence analyses, a new type of PCV was identified in those three cases. Based on sequence analyses, this new PCV genome contains the ORF1 of PCV1 and the ORF2 of PCV2a and its entire viral genome nucleotide identity compared to PCV1, PCV2a and 2b are 86.4%, 88.7% and 86.5%, respectively. It is proposed to name this new PCV by taking into account the nomenclature of Segales et al. (2008) and by indicating the origin of the ORF1 at first and the origin of the ORF2 in second. Consequently, the name proposed for this new PCV is PCV1/2a. The prevalence of PCV1/2a seems to be very low in Quebec, Canada (2.5% of PCV positive cases), and its origin is now in debate. (C) 2010 Elsevier B.V. All rights reserved.