Near-infrared light induced in vivo photodynamic therapy of cancer based on upconversion nanoparticles
Near-infrared light induced in vivo photodynamic therapy of cancer based on upconversion nanoparticles
复制标题
基于上转换纳米粒子的近红外光诱导体内癌症光动力疗法
DOI：
10.1016/j.biomaterials.2011.05.007
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发表时间：
2011-09-01
期刊：
影响因子：
14
通讯作者：
Liu, Zhuang
中科院分区：
文献类型：
--
作者：
Wang, Chao;Tao, Huiquan;Liu, Zhuang
上转换纳米粒子（UCNPs）在低能量近红外（NIR）光的激发下发射高能光子，是一种新型的生物医学光学纳米探针。本文中，我们将光敏剂二氢卟酚e6（Ce 6）负载在聚合物包覆的UCNPs上，形成UCNP-Ce 6超分子复合物，其在NIR光下产生单线态氧以杀死癌细胞。在肿瘤内注射UCNP-Ce 6和随后的NIR光暴露后，在荷瘤小鼠中实现了优异的光动力疗法（PDT）功效。进一步揭示了PDT处理后的UCNPs逐渐从小鼠器官中清除，而不会对经处理的动物产生明显的毒性。此外，我们证明了基于UCNP-Ce 6的NIR诱导的PDT与使用可见激发光的传统PDT相比表现出显著增加的组织穿透深度，为厚生物组织阻断的肿瘤提供显著改善的治疗效果。我们的工作证明了近红外光诱导的动物体内癌症PDT治疗，并突出了UCNPs用于多功能体内癌症治疗和成像的前景。(C)2011爱思唯尔有限公司保留所有权利。
Upconversion nanoparticles (UCNPs) that emit high-energy photons upon excitation by the low-energy near-infrared (NIR) light are emerging as new optical nano-probes useful in biomedicine. Herein, we load Chlorin e6 (Ce6), a photosensitizer, on polymer-coated UCNPs, forming a UCNP-Ce6 supramolecular complex that produces singlet oxygen to kill cancer cells under NIR light. Excellent photodynamic therapy (PDT) efficacy is achieved in tumor-bearing mice upon intratumoral injection of UCNP-Ce6 and the followed NIR light exposure. It is further uncovered that UCNPs after PDT treatment are gradually cleared out from mouse organs, without rendering appreciable toxicity to the treated animals. Moreover, we demonstrate that the NIR-induced PDT based on UCNP-Ce6 exhibits a remarkably increased tissue penetration depth compared to the traditional PDT using visible excitation light, offering significantly improved treatment efficacy for tumors blocked by thick biological tissues. Our work demonstrates NIR light-induced in vivo PDT treatment of cancer in animals, and highlights the promise of UCNPs for multifunctional in vivo cancer treatment and imaging. (C) 2011 Elsevier Ltd. All rights reserved.