Improved Method of Porcine Pancreas Procurement With Arterial Flush and Ductal Injection Enhances Islet Isolation Outcome
Improved Method of Porcine Pancreas Procurement With Arterial Flush and Ductal Injection Enhances Islet Isolation Outcome
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DOI：
10.1016/j.transproceed.2010.05.110
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发表时间：
2010-07-01
影响因子：
0.9
通讯作者：
Hering, B. J.
中科院分区：
文献类型：
--
作者：
Anazawa, T.;Balamurugan, A. N.;Hering, B. J.
背景几种胰腺采购程序已用于猪胰岛分离;然而，其对结果的影响尚未得到广泛研究。我们评估了一种用于猪胰岛分离的先进采购技术，该技术旨在通过实施血管冲洗和导管扩张来减少热缺血、去除血液内容物并增强胰腺的冷却。从成年长白猪获得的胰腺分为3种不同的手术方案：仅利用表面冷却的胰腺切除术（组1; n = 24）;胰腺切除术前表面冷却和胰管注射冷保存液（组2; n = 12）;或表面冷却、导管注射和通过腹腔干和上级肠系膜动脉选择性冲洗的方法（第3组，n = 14）。我们使用体外和体内试验评估胰岛分离的结果和质量。与其他组相比，第3组猪的总产量和每克胰腺的胰岛产量显著更高。通过每个DNA的耗氧率评估的培养7天后胰岛活力的测量显示，第3组胰岛显示出最高值。在移植了来自所有3组的2000个胰岛当量的糖尿病裸小鼠中观察到持续的血糖正常。讨论。本研究表明，先进的胰腺获取技术，包括导管保存和选择性动脉冲洗冷保存液提供了显着改善猪胰岛分离的结果。
Background. Several pancreas procurement procedures have been used for porcine islet isolation; however, their impact on outcomes has not been extensively studied. We evaluated an advanced procurement technique for porcine islet isolation designed to reduce warm ischemia, to remove blood content, and enhance cooling of the pancreas by implementing a vascular flush and ductal preservation.Method. Pancreata procured from adult Landrace pigs were divided into 3 different surgical protocols: Pancreatectomy utilizing only surface cooling (group 1; n = 24); surface cooling and ductal injection with cold preservation solution before pancreatectomy (group 2; n = 12); or surface cooling, ductal injection, and an approach by selectively flushing through the celiac trunk and the superior mesenteric artery (group 3; n = 14). We assessed the islet isolation results and quality using in vitro and in vivo assays.Results. Significantly higher overall yield and islet yield per gram pancreas were obtained from group 3 pigs compared with the other groups. Measurements of islet viability after 7 days of culture, as assessed by oxygen consumption rate per DNA, showed that group 3 islets displayed the highest values. Sustained normoglycemia was observed in diabetic nude mice transplanted with 2000 islet equivalents from all 3 groups.Discussion. This study demonstrated that an advanced pancreas procurement technique including ductal preservation and selective arterial flush with cold preservation solution provided significant improvements in porcine islet isolation outcomes.