Cellular image analysis and imaging by flow cytometry
Cellular image analysis and imaging by flow cytometry
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DOI：
10.1016/j.cll.2007.05.008
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发表时间：
2007-09-01
影响因子：
1.7
通讯作者：
Morrissey, Philip
中科院分区：
文献类型：
--
作者：
Basiji, David A.;Ortyn, William E.;Morrissey, Philip
成像流式细胞术将标准流式细胞术的统计能力和荧光灵敏度与数字显微镜的空间分辨率和定量形态相结合。该技术提供了一种直接对体液中的细胞进行成像和分析的便捷方法，非常适合临床应用。提供了区分癌性和正常乳腺上皮细胞以及对人外周血单核细胞中荧光原位杂交 (FISH) 探针进行高通量定量的示例。通过扩展景深成像技术与当前光学系统的集成，FISH 应用将得到进一步增强。
Imaging flow cytometry combines the statistical power and fluorescence sensitivity of standard flow cytometry with the spatial resolution and quantitative morphology of digital microscopy. The technique is a good fit for clinical applications by providing a convenient means for imaging and analyzing cells directly in bodily fluids. Examples are provided of the discrimination of cancerous from normal mammary epithelial cells and the high-throughput quantitation of fluorescence in situ hybridization (FISH) probes in human peripheral blood mononuclear cells. The FISH application will be enhanced further by the integration of extended depth-of-field imaging technology with the current optical system.