Zwitterionic polymers exhibiting high resistance to nonspecific protein adsorption from human serum and plasma
Zwitterionic polymers exhibiting high resistance to nonspecific protein adsorption from human serum and plasma
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DOI：
10.1021/bm701301s
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发表时间：
2008-05-01
影响因子：
6.2
通讯作者：
Jiang, Shaoyi
中科院分区：
文献类型：
--
作者：
Ladd, Jon;Zhang, Zheng;Jiang, Shaoyi
本研究考察了六种不同的聚合物和自组装单层（SAM）表面修饰与人血清和血浆的相互作用。结果表明，两性离子聚合物表面是可行的替代更传统的表面的基础上聚（乙二醇）（PEG）作为非污垢表面。所有的聚合物表面形成使用原子转移自由基聚合（ATRP），它们表现出增加的抵抗非特异性蛋白质吸附相比，自组装膜。这种改进是由于在表面上的非污垢基团的表面堆积密度的增加，以及从柔性聚合物刷表面的空间排斥。基于羧基甜菜碱甲基丙烯酸酯（CBMA）的两性离子聚合物表面还结合了用于在无污染背景下固定蛋白质的官能团，使其成为许多应用的强有力的候选者，例如在诊断和药物递送中。
This study examined six different polymer and self-assembled monolayer (SAM) surface modifications for their interactions with human serum and plasma. It was demonstrated that zwitterionic polymer surfaces are viable alternatives to more traditional surfaces based on poly(ethylene glycol) (PEG) as nonfouling surfaces. All polymer surfaces were formed using atom transfer radical polymerization (ATRP) and they showed an increased resistance to nonspecific protein adsorption compared to SAMs. This improvement is due to an increase in the surface packing density of nonfouling groups on the surface, as well as a steric repulsion from the flexible polymer brush surfaces. The zwitterionic polymer surface based on carboxybetaine methacrylate (CBMA) also incorporates functional groups for protein immobilization in the nonfouling background, making it a strong candidate for many applications such as in diagnostics and drug delivery.