Spatiotemporal expression pattern of Mirg, an imprinted non-coding gene, during mouse embryogenesis
Spatiotemporal expression pattern of Mirg, an imprinted non-coding gene, during mouse embryogenesis
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印迹非编码基因 Mirg 在小鼠胚胎发生过程中的时空表达模式
DOI：
10.1007/s10735-011-9367-x
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发表时间：
2012-02-01
影响因子：
3.2
通讯作者：
Wu, Qiong
中科院分区：
文献类型：
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作者：
Han, Zhengbin;He, Hongjuan;Wu, Qiong
最近的研究表明，Dlk1-dio3印迹簇中的母体非编码RNA基因(Gtl2、Rian和Mirg)与诱导的多能干细胞(IPSCs)的全部发育潜力密切相关。这些基因的转录沉默未能产生ALL-IPSC小鼠，表明它们对胚胎发育有重大贡献。然而，除了Gtl2外，在这个簇中几乎没有获得关于这些基因的信息。在本研究中，我们分析了Mirg在小鼠胚胎发育过程中的时空表达模式。利用原位杂交和定量聚合酶链式反应，我们证明了Mirg非编码RNA在小鼠胚胎发育过程中持续表达，从E8.5到E18.5。在中枢神经系统(E9.5-E15.5)和各种骨骼肌(E13.5和E15.5)有较强的表达，亚细胞定位于细胞核。垂体和肾上腺也显示出Mirg的高表达，但与骨骼肌和神经回路不同的是，这些信号并不集中在细胞核中。在主要内脏器官中，Mirg在胚胎发育过程中保持低表达(E12.5-E18.5)，而在肝脏和发育中的肺中，Mirg的表达分别呈逐渐减少和逐渐升高的趋势。这些发现表明，在胚胎发育的特定阶段和特定组织中，可能需要对Mirg的表达进行时间调节。
Recent research has revealed that the maternal non-coding RNA genes (Gtl2, Rian and Mirg) from the Dlk1-Dio3 imprinted cluster are closely related to the full development potential of the induced pluripotent stem cells (iPSCs). Transcriptional silencing of these genes failed to generate all-iPSC mice, indicating their significant contribution to embryogenesis. However, except for Gtl2, little information regarding these genes has been acquired in this cluster. In the present study, we analyzed the spatiotemporal expression patterns of Mirg during mouse embryogenesis. Using in situ hybridization and quantitative PCR, we demonstrated that Mirg non-coding RNA exhibited sustained expression throughout mouse embryogenesis from E8.5 to E18.5. Strong expression was detected in the central nervous system (E9.5–E15.5) and various skeletal muscles (E13.5 and E15.5), and the subcellular localization appeared to be in the nuclei. The pituitary and adrenal gland also showed high expression of Mirg, but, unlike the skeletal muscles and the neural circuitry, the signals were not concentrated in the nuclei. In the major internal organs, Mirg maintained low expression during embryogenesis (E12.5–E18.5) whereas in the liver and the developing lung, Mirg was expressed with a gradually decreasing trend and a gradually raising trend, respectively. These findings indicate that temporal regulation of Mirg expression may be required during specific stages and in specific tissues during embryonic development.