growth and for cytokinesis in budding yeast. Ste20-like protein kinases are required for normal localization of cell
growth and for cytokinesis in budding yeast. Ste20-like protein kinases are required for normal localization of cell
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发表时间：
2007
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通讯作者：
Fatima Cvr;E. Manser;J. Pringle
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作者：
Fatima Cvr;E. Manser;J. Pringle
酵母菌Ste20蛋白激酶参与信息素反应。哺乳动物中存在Ste20的同源物，但它们的功能尚不清楚。我们鉴定了一种新的酵母STE20同源物，CLA4，在缺乏G1周期蛋白Cln1和Cln2的情况下筛选出致命的突变。Cla4参与出芽和胞质分裂，并与CDC42相互作用，CDC42是极化细胞生长所需的GTP酶。尽管存在胞质分裂缺陷，Cla4突变体仍然有效。然而，双爪4ste20突变体不能维持花蕾颈部的隔膜环，也不能进行细胞质分裂。在同一筛查中发现了编码其中一种分隔素的CDC12的突变。Cla4和Ste20蛋白激酶显然在细胞生长定位中与隔素环有共同作用。
The yeast Ste20 protein kinase is involved in pheromone response. Mammalian homologs of Ste20 exist, but their function remains unknown. We identified a novel yeast STE20 homolog, CLA4, in a screen for mutations lethal in the absence of the G1 cyclins Clnl and Cln2. Cla4 is involved in budding and cytokinesis and interacts with Cdc42, a GTPase required for polarized cell growth. Despite a cytokinesis defect, cla4 mutants are viable. However, double cla4 ste20 mutants cannot maintain septin rings at the bud neck and cannot undergo cytokinesis. Mutations in CDC12, which encodes one of the septins, were found in the same screen. Cla4 and Ste20 kinases apparently share a function in localizing cell growth with respect to the septin ring.