Piperine inhibit inflammation, alveolar bone loss and collagen fibers breakdown in a rat periodontitis model
Piperine inhibit inflammation, alveolar bone loss and collagen fibers breakdown in a rat periodontitis model
复制标题
胡椒碱抑制大鼠牙周炎模型中的炎症、牙槽骨丢失和胶原纤维分解
DOI：
10.1111/jre.12262
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发表时间：
2015-12-01
影响因子：
3.5
通讯作者：
Li, C.
中科院分区：
文献类型：
--
作者：
Dong, Y.;Huihui, Z.;Li, C.
背景和研究表明，苦参碱具有抗炎活性，并且具有悠久的药用历史。本研究旨在探讨胡椒碱对实验性牙周炎炎症、牙槽骨和胶原纤维的保护作用。我们评估了白细胞介素（IL）-1，肿瘤坏死因子（TNF）-α，基质金属蛋白酶（MMP）-8和MMP-13的相关表达，以提高洞察到这些effects.Material和Methods 32只Wistar大鼠分为4组，每组8只动物：对照组，牙周炎组，牙周炎加50 mg/kg胡椒碱组和牙周炎加100 mg/kg胡椒碱组。苏木精-伊红染色观察组织学改变。通过微型计算机断层扫描评估牙槽骨丢失和骨小梁显微结构。通过天狼星红染色评估胶原纤维的变化。Western blot检测IL-1、TNF-α、MMP-8和MMP-13的表达。结果苦参碱可明显抑制牙槽骨丢失，并呈剂量依赖性地促进牙槽骨小梁的重建。组织学染色显示胡椒碱明显减少软组织炎症浸润。两种剂量的胡椒碱都限制了胶原纤维中降解区域的分数。结果表明：100 mg/kg苦参碱可显著下调IL-1、MMP-8和MMP-13的表达，但对TNF-α的表达无明显影响。结论苦参碱对实验性牙周炎炎症反应、牙槽骨丢失、骨微结构和胶原纤维降解具有保护作用。其作用可能与抑制IL-1、MMP-8和MMP-13的表达有关。
Background and ObjectivePiperine exhibits anti-inflammatory activity, and has a long history of medicinal use. The objective of this study was to investigate the protective effects of piperine on inflammation, alveolar bone and collagen fibers in experimental periodontitis. We evaluated the related expression of interleukin (IL)-1, tumor necrosis factor (TNF)-, matrix metalloproteinase (MMP)-8 and MMP-13 to enhance insight into these effects.Material and MethodsThirty-two Wistar rats were divided into four groups of eight animals each: control group, periodontitis group, periodontitis plus 50mg/kg piperine group and periodontitis plus 100mg/kg piperine group. Histopathologic changes were detected by hematoxylin and eosin staining. Alveolar bone loss and trabecula microstructures were evaluated by micro-computed tomography. Changes in collagen fibers were assessed by picrosirius red staining. Western blot analysis was conducted to determine the levels of IL-1, TNF-, MMP-8 and MMP-13.ResultsPiperine clearly inhibited alveolar bone loss and reformed trabecula microstructures in a dose-dependent manner. Histological staining showed that piperine significantly reduced the infiltration of inflammation in soft tissues. Both doses of piperine limited the fractions of degraded areas in collagen fibers. Piperine (100mg/kg) significantly downregulated the expressions of IL-1, MMP-8 and MMP-13 in periodontitis, but not that of TNF-.ConclusionPiperine displays significantly protective effects on inflammation, alveolar bone loss, bone microstructures and collagen fiber degradation in experimental periodontitis. The effects may be ascribed to its inhibitory activity on the expressions of IL-1, MMP-8 and MMP-13.