COMPARTMENTALIZATION OF MILK N-ACETYL-BETA-D-GLUCOSAMINIDASE (NAGASE) - RELEASE OF NAGASE FROM THE CELLULAR COMPARTMENT BY STORAGE, FREEZING AND THAWING, DETERGENT AND USING CELL STIMULANTS
COMPARTMENTALIZATION OF MILK N-ACETYL-BETA-D-GLUCOSAMINIDASE (NAGASE) - RELEASE OF NAGASE FROM THE CELLULAR COMPARTMENT BY STORAGE, FREEZING AND THAWING, DETERGENT AND USING CELL STIMULANTS
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DOI：
10.1111/j.1439-0450.1988.tb00513.x
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发表时间：
1988-07-01
期刊：
影响因子：
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通讯作者：
SANDHOLM, M
中科院分区：
文献类型：
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作者：
KAARTINEN, L;KUOSA, PL;SANDHOLM, M
约55%的溶酶体酶n -乙酰基。新采集的乳乳汁中的- d -氨基葡萄糖酶（NAGase）存在于细胞区室、离心机或顶部奶油层中。在健康牛奶中，这一比例甚至更高，达到94%。在储存过程中，通过用内毒素或调理酶刺激细胞，通过冷冻和解冻循环，将长酶从细胞室中释放出来。在牛奶样品中加入2%的Triton X-100可获得最大的活性释放。NAGase分析要求牛奶样品的取样和处理标准化。监测NAGase在脱脂牛奶中的释放被认为是分析细胞活化的一种方法。
About 55% of the lysosomal enzyme N-acetyl-.beta.-D-glucosaminidase (NAGase) in newly collected mastitic milk was present within the cellular compartments, the centrifugate or the top cream layer. In milk from healthy quarters this percentage was even higher, 94%. The NAGase was released from the cellular compartments upon storage, a cycle of freezing and thawing, by stimulating cells with endotoxin or opsonized zymosan. Maximum release of activity was obtained by including 2% Triton X-100 in the milk samples. NAGase analysis requires standardization of the sampling and handling of milk samples. Monitoring the release of NAGase into the skimmed milk fraction is suggested as a method for analyzing cellular activation.