The centromeric sister chromatid cohesion site directs Mcd1p binding to adjacent sequences
The centromeric sister chromatid cohesion site directs Mcd1p binding to adjacent sequences
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DOI：
10.1016/s1097-2765(00)80347-0
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发表时间：
1999-09-01
期刊：
影响因子：
16
通讯作者：
Koshland, D
中科院分区：
文献类型：
--
作者：
Megee, PC;Mistrot, C;Koshland, D
姐妹染色单体之间的粘合发生在染色体的长度上，在染色体分离中起着至关重要的作用。我们在这里表明，着丝粒是一种CIA作用的凝聚力因子，它以一种独立于序列的方式引导粘附素亚单位Mcd1p与着丝粒两侧至少2kb的区域结合。着丝粒对于维持和建立这个粘连蛋白结构域是必不可少的。Mcd1p在粘附素结构域内的结合效率与着丝粒侧翼DNA的初级核苷酸序列无关，但与高A+T DNA含量有关。因此，着丝粒在着丝粒-近端区域的凝聚中的功能可能类似于增强子的功能，在A+T丰富的DNA的扩展染色体结构域上形成结合的粘附素复合体。
Cohesion between sister chromatids occurs along the length of chromosomes, where it plays essential roles in chromosome segregation. We show here that the centromere, a cia-acting cohesion factor, directs the binding of Mcd1p, a cohesin subunit, to at least 2 kb regions flanking centromeres in a sequence-independent manner. The centromere is essential for the maintenance as well as the establishment of this cohesin domain. The efficiency of Mcd1p binding within the cohesin domain is independent of the primary nucleotide sequence of the centromere-flanking DNA but correlates with high A+T DNA content. Thus, the function of centromeres in the cohesion of centromere-proximal regions may be analogous to that of enhancers, nucleating cohesin complex binding over an extended chromosomal domain of A+T-rich DNA.