Combinatorial control required for the specificity of yeast MAPK signaling
Combinatorial control required for the specificity of yeast MAPK signaling
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DOI：
10.1126/science.275.5304.1314
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发表时间：
1997-02-28
期刊：
影响因子：
56.9
通讯作者：
Fink, GR
中科院分区：
文献类型：
--
作者：
Madhani, HD;Fink, GR
在酵母中，一组重叠的丝裂原活化蛋白激酶(MAPK)信号元件控制交配、单倍体入侵和假菌丝发育。矛盾的是，单个下游转录因子STE12是执行这些不同程序所必需的。发育特异性需要TEA/ATTS家族的转录因子Tec1，它在丝状和侵袭性生长过程中与STE12合作。STE12和Tec1的纯化衍生物协同结合到称为丝状化和侵袭反应元件(FRES)的增强子元件上，FRES对转录进行编程，专门对丝状细胞生长所需的MAPK信号成分做出反应。TEC1启动子中的FRE在假菌丝发育所需的正反馈环中发挥作用。
In yeast, an overlapping set of mitogen-activated protein kinase (MAPK) signaling components controls mating, haploid invasion, and pseudohyphal development. Paradoxically, a single downstream transcription factor, Ste12, is necessary for the execution of these distinct programs. Developmental specificity was found to require a transcription factor of the TEA/ATTS family, Tec1, which cooperates with Ste12 during filamentous and invasive growth. Purified derivatives of Ste12 and Tec1 bind cooperatively to enhancer elements called filamentation and invasion response elements (FREs), which program transcription that is specifically responsive to the MAPK signaling components required for filamentous growth. An FRE in the TEC1 promoter functions in a positive feedback loop required for pseudohyphal development.