Pol V produced RNA facilitates transposable element excision site repair in Arabidopsis.
Pol V produced RNA facilitates transposable element excision site repair in Arabidopsis.
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DOI：
10.17912/micropub.biology.000793
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发表时间：
2023
影响因子：
--
通讯作者：
Hancock, C Nathan
中科院分区：
文献类型：
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作者：
Renken, Kaili;Mendoza, Sarah M;Diaz, Stephanie;Slotkin, R Keith;Hancock, C Nathan
植物特有的RNA聚合酶V(PolV)在基因沉默中起着关键作用，但其在双链DNA断裂修复中的作用尚不清楚。切除转座元件mPING会产生由NHEJ修复的双链断裂。我们使用mPING：GFP报告基因在包括polV在内的多个DNA甲基化突变体中测量了mPING切除位点修复。POL V的两个独立突变等位基因GFP表达较低，提示POL V蛋白在切除部位修复中起作用。对polV切除位点的序列分析表明，较大的缺失率升高，修复效率较低。这些结果阐明了Pol V在双链DNA断裂修复中的作用，而不是其他RNA指导的DNA甲基化蛋白(Pol IV)或维持DNA甲基化途径(MET1)。
The plant-specific RNA Polymerase V (Pol V) plays a key role in gene silencing, but its role in repair of double stranded DNA breaks is unclear. Excision of the transposable element mPing creates double stranded breaks that are repaired by NHEJ. We measured mPing excision site repair in multiple DNA methylation mutants including pol V using an mPing : GFP reporter. Two independent mutant alleles of pol V showed less GFP expression, indicating that the Pol V protein plays a role in excision site repair. Sequence analysis of the pol V excision sites indicated an elevated rate of large deletions consistent with less efficient repair. These results clarify the role of Pol V, but not other RNA-directed DNA methylation proteins (Pol IV) or maintenance DNA methylation pathways ( MET1 ), in the repair of double-strand DNA breaks.