THE ROLE OF 20-HYDROXY-ECDYSONE IN YOLK-POLYPEPTIDE SYNTHESIS BY MALE AND FEMALE FAT-BODIES OF DROSOPHILA-MELANOGASTER
THE ROLE OF 20-HYDROXY-ECDYSONE IN YOLK-POLYPEPTIDE SYNTHESIS BY MALE AND FEMALE FAT-BODIES OF DROSOPHILA-MELANOGASTER
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DOI：
10.1016/0022-1910(82)90043-9
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发表时间：
1982-01-01
影响因子：
2.2
通讯作者：
BOWNES, M
中科院分区：
文献类型：
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作者：
BOWNES, M
果蝇的雄性成虫通常不产生卵黄多肽，但 20-羟基-蜕皮激素会刺激其产生卵黄多肽。对单剂量激素的反应是脂肪体短暂爆发卵黄多肽合成。反应的强度取决于剂量。第二次激素治疗会产生类似的反应。长期喂养雄性不会导致卵黄多肽基因的永久激活。体外用20-羟基蜕皮激素不可能刺激雄性脂肪体合成卵黄多肽。 20-羟基-蜕皮激素，可能在另一种激素或载体蛋白存在的情况下，诱导男性脂肪体的卵黄多肽基因的转录，并且它的存在是保持基因活性所必需的。在女性中，成熟的脂肪体可以在体外产生卵黄多肽，而不需要添加激素。男性和女性脂肪体对于维持编码卵黄多肽的基因的表达具有不同的激素需求。
Male adults of Drosophila, which do not normally produce yolk polypeptides are stimulated to do so by 20-hydroxy-ecdysone. The response to a single dose of hormone is a short burst of yolk-polypeptide synthesis by the fat body. The magnitude of the response is dose dependent. A 2nd treatment with the hormone leads to a similar response. Long-term feeding of males does not lead to permanent activation of the yolk-polypeptide genes. It was not possible to stimulate male fat bodies to synthesize yolk polypeptides with 20-hydroxy-ecdysone in vitro. 20-Hydroxy-ecdysone, possibly in the presence of another hormone or a carrier protein, induces transcription of the yolk-polypeptide genes of the male fat body and its presence is required to keep the genes active. In females mature fat bodies can produce yolk polypeptides in vitro without the requirement for added hormone. Male and female fat bodies have different hormonal requirements for the maintenance of expression of the genes coding for the yolk polypeptides.