Postmortem delay does not change regional diffusion anisotropy characteristics in mouse spinal cord white matter
Postmortem delay does not change regional diffusion anisotropy characteristics in mouse spinal cord white matter
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DOI：
10.1002/nbm.1138
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发表时间：
2007-05-01
影响因子：
2.9
通讯作者：
Song, Sheng-Kwei
中科院分区：
文献类型：
--
作者：
Kim, Joong Hee;Trinkaus, Kathryn;Song, Sheng-Kwei
已经证明，水在体内的扩散各向异性相当于小鼠脑内灌流固定后在体外观察到的各向异性。这一发现支持对灌注固定的组织进行体外扩散张量成像(DTI)测量的实践。然而，将浸泡固定的身体标本的体外DTI测量外推到活体状态的有效性是值得怀疑的，因为固定前经常遇到可变的死后延迟。在这项研究中，我们研究了死后延迟对小鼠脊髓腹外侧白质水扩散各向异性的影响。取同一种动物的小鼠脊髓，分别在活体、死后固定前原位、死后10h固定的体外浸泡状态下进行DTI检查。我们的结果表明，小鼠脊髓的扩散各向异性在死亡后10小时内仍保持不变。小鼠脊髓白质弥散各向异性的区域性特征在体内、死后原位(直至固定前10h)和浸泡固定后15周的体外相同。版权所有(C)2007 John Wiley&Sons，Ltd.
It has been demonstrated previously that water diffusion anisotropy in vivo is equivalent to that observed ex vivo after perfusion fixation in the mouse brain. This finding supports the practice of ex vivo diffusion tensor imaging (DTI) measurement on perfusion-fixed tissues. However, the validity of extrapolating ex vivo DTI measurements from immersion-fixed autopsy specimens to the in vivo state is questionable because of variable postmortem delays often encountered before fixation. In this study, we investigated the effect of postmortem delay on the water diffusion anisotropy of ventrolateral spinal cord white matter from mice. Mouse spinal cords, each from the same animal, were examined using DTI in vivo, in situ after death before fixation, and ex vivo immersion fixed 10 h after death. Our results suggest that diffusion anisotropy in mouse spinal cord is preserved up to 10 h after death. Regional characteristics of diffusion anisotropy in mouse spinal cord white matter are equivalent in vivo, in situ after death (up to 10 h before fixation), and ex vivo 15 weeks after immersion fixation. Copyright (C) 2007 John Wiley & Sons, Ltd.