Rhabdopeptides as Insect-Specific Virulence Factors from Entomopathogenic Bacteria
Rhabdopeptides as Insect-Specific Virulence Factors from Entomopathogenic Bacteria
复制标题
DOI：
10.1002/cbic.201300205
复制
发表时间：
2013-10-11
期刊：
影响因子：
3.2
通讯作者：
Bode, Helge B.
中科院分区：
文献类型：
--
作者：
Reimer, Daniela;Cowles, Kimberly N.;Bode, Helge B.
利用启动子陷阱检测昆虫诱导基因，在嗜线虫Xenorhabdus nematophila中发现了相应的rdp基因簇，并鉴定出6个新的线状肽，命名为rhabdopeptides。这些横纹肌多肽的结构是通过标记实验结合详细的质谱分析推断出来的。对rdp突变体的详细分析表明，这些化合物参与对昆虫的毒力，并在细菌感染合适的昆虫宿主后产生。此外，我们还分离出了另外两种由卡巴尼罗Xenorhabdus cabanillasii产生的横纹肌肽衍生物，它们显示出对昆虫血细胞的活性，从而证实了这类新化合物的毒力。
Six novel linear peptides, named rhabdopeptides, have been identified in the entomopathogenic bacterium Xenorhabdus nematophila after the discovery of the corresponding rdp gene cluster by using a promoter trap strategy for the detection of insect-inducible genes. The structures of these rhabdopeptides were deduced from labeling experiments combined with detailed MS analysis. Detailed analysis of an rdp mutant revealed that these compounds participate in virulence towards insects and are produced upon bacterial infection of a suitable insect host. Furthermore, two additional rhabdopeptide derivatives produced by Xenorhabdus cabanillasii were isolated, these showed activity against insect hemocytes thereby confirming the virulence of this novel class of compounds.