Real-time monitoring of lipid transfer between vesicles and hybrid bilayer on Au nanoshells using surface enhanced Raman scattering (SERS)
Real-time monitoring of lipid transfer between vesicles and hybrid bilayer on Au nanoshells using surface enhanced Raman scattering (SERS)
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DOI：
10.1039/b9nr00063a
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发表时间：
2009-10-01
期刊：
影响因子：
6.7
通讯作者：
Halas, Naomi J.
中科院分区：
文献类型：
--
作者：
Kundu, Janardan;Levin, Carly S.;Halas, Naomi J.
为了研究膜之间脂质交换/转移的动力学，我们使用Sers研究了供体氘代DMPC囊泡与Au纳米壳上DMPC混合双层的相互作用。实验数据证实部分脂质交换/转移的混合双层的外叶。交换/转移过程的动力学遵循速率常数为1.3 × 10（-4）s（-1）的一级过程。脂质相行为的变化所造成的交换/转移过程中，其特征在于使用广义偏振测量。原位脂质转移可用于制备不对称支撑的脂质双层和将报告脂质掺入生物膜中。
To investigate the dynamics of exchange/transfer of lipids between membranes, we have studied the interaction of donor-deuterated DMPC vesicles with DMPC hybrid bilayers on Au nanoshells using SERS. Experimental data confirm partial lipid exchange/transfer in the outer leaflet of the hybrid bilayer. The kinetics of the exchange/transfer process follows a first order process with a rate constant of 1.3 x 10(-4) s(-1). Changes in lipid phase behavior caused by the exchange/transfer process were characterized using generalized polarization measurements. In situ lipid transfer can potentially be utilized for preparation of asymmetric supported lipid bilayers and for incorporation of reporter lipids in biological membranes.