EFFECTS OF ACARBOSE ON STARCH HYDROLYSIS - STUDY IN HEALTHY-SUBJECTS, ILEOSTOMY PATIENTS, AND INVITRO
EFFECTS OF ACARBOSE ON STARCH HYDROLYSIS - STUDY IN HEALTHY-SUBJECTS, ILEOSTOMY PATIENTS, AND INVITRO
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DOI：
10.1007/bf01300287
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发表时间：
1992-07-01
影响因子：
3.1
通讯作者：
VANTRAPPEN, G
中科院分区：
文献类型：
--
作者：
HIELE, M;GHOOS, Y;VANTRAPPEN, G
在正常受试者和回肠造口患者中，通过 (CO2)-C-13 呼气试验和血糖水平作为吸收参数，以及 H-2 呼气试验、血清乙酸水平和回肠碳水化合物损失作为吸收不良参数，研究阿卡波糖对纯淀粉粉水解的影响。通过体外实验获得了关于阿卡波糖对 α-淀粉酶活性影响的更多信息。阿卡波糖（200 和 400 毫克）显着延迟淀粉吸收。研究发现，与呼吸 H-2 排泄相比，血清醋酸盐作为吸收不良标志物的敏感性较低。摄入50克淀粉加400毫克阿卡波糖后，23-71%的淀粉负荷在回肠造口流出物中损失，其中大部分以淀粉形式损失。这表明阿卡波糖显着抑制α-淀粉酶，而不仅仅是刷状缘酶。体外实验证实，药理学使用剂量的阿卡波糖可预期抑制三分之二的α-淀粉酶活性。
The effect of acarbose on hydrolysis of a pure starch meal was investigated in normal subjects and ileostomy patients by means of (CO2)-C-13 breath tests and blood glucose levels as parameters of absorption, and of H-2 breath tests, serum acetate levels, and ileal loss of carbohydrate as parameters of malabsorption. Additional information on the effect of acarbose on alpha-amylase activity was obtained by in vitro experiments. Acarbose (200 and 400 mg) significantly delayed starch absorption. Serum acetate was found to be a less sensitive marker of malabsorption than breath H-2 excretion. After intake of 50 g starch plus 400 mg acarbose, 23-71% of the starch load was lost in the ileostomy effluent, for a large part as starch. This suggests that acarbose considerably inhibits alpha-amylase, and not only brush-border enzymes. In vitro experiments confirm that an inhibition of two thirds of alpha-amylase activity can be expected from pharmacologically used doses of acarbose.