A region in the CD8 gene locus that directs expression to the mature CD8 T cell subset in transgenic mice
A region in the CD8 gene locus that directs expression to the mature CD8 T cell subset in transgenic mice
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DOI：
10.1016/s1074-7613(00)80374-x
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发表时间：
1997-10-01
期刊：
影响因子：
32.4
通讯作者：
Kioussis, D
中科院分区：
文献类型：
--
作者：
Hostert, A;Tolaini, M;Kioussis, D
辅助受体CD 4和CD 8在胸腺细胞发育和T细胞效应功能中起着至关重要的作用，并且它们的表达受到发育调节。为了确定CD 8基因调控的潜在分子机制，我们从基因组文库中克隆了小鼠CD 8基因位点，并分析了该区域的脱氧核糖核酸酶（DNase I）超敏位点（HSS）。在这里，我们报告，使用转基因小鼠，删除分析的一个确定的集群的DNase I超敏反应，由三个DNase I-HSS和位于基因间区域之间的CD 8 α和CD 8 β基因。我们的数据表明，至少有两个DNA酶I-HSS构成这个集群是单独足以指导CD 8 α或异源转基因表达的成熟的CD 8单阳性T细胞亚群，这种表达时间上符合阳性选择的T细胞的外观。
The coreceptors CD4 and CD8 play a crucial role during thymocyte development and T cell effector function, and their expression is developmentally regulated. To determine the underlying molecular mechanisms of CD8 gene regulation we cloned the murine CD8 gene locus from genomic libraries and analyzed this region for deoxyribonuclease (DNase I) hypersensitive sites (HSS). Here we report, using transgenic mice, deletion analysis of one of the identified clusters of DNase I hypersensitivity, consisting of three DNase I-HSS and located in the intergenic region between the CD8 alpha and CD8 beta genes. Our data show that at least two of the DNase I-HSS constituting this cluster are individually sufficient to direct CD8 alpha or heterologous transgene expression to the mature CD8 single-positive T cell subset and that this expression coincides temporally with the appearance of positively selected T cells.