ISCHEMIC TIME-DEPENDENT MICROVASCULAR CHANGES AND REPERFUSION INJURY IN THE RAT SMALL-INTESTINE
ISCHEMIC TIME-DEPENDENT MICROVASCULAR CHANGES AND REPERFUSION INJURY IN THE RAT SMALL-INTESTINE
复制标题
DOI：
10.1006/jsre.1995.1170
复制
发表时间：
1995-08-01
影响因子：
2.2
通讯作者：
HATANAKA, K
中科院分区：
文献类型：
--
作者：
BOROS, M;TAKAICHI, S;HATANAKA, K
再灌注期在肠循环完全闭塞的病理生理学中的意义是有争议的。我们的研究旨在评估再灌注诱导的肠粘膜损伤的确切幅度在一个标准化的大鼠模型的完全节段性动脉缺血作为闭塞时间的函数。肠缺血维持15，30，或60分钟，或缺血后30分钟的再灌注期。采用墨汁灌注法观察缺血或缺血再灌注引起的黏膜血管变化。通过图像分析系统记录绒毛分布小动脉的高度和平均粘膜厚度，并以半定量0 - 5级量表确定粘膜损伤程度。缺血诱导红细胞阻塞在绒毛尖端，碳填充的小动脉高度的进行性降低，并伴随着7%，23%，或35%的粘膜厚度减少，灌注的小动脉高度/粘膜厚度减少百分比的百分比减少率分别为2.2%，1.5%，或1，在15-，30-，或60-min缺血。缺血60分钟组可见碳示踪剂外渗，再灌注时粘膜层分别较缺血前减少27%、38%和57%。所有缺血再灌注组的小动脉高度降低/粘膜厚度降低比率均为1：1。缺血15分钟后再灌注时粘膜损伤程度明显加重。微血管阻塞在动脉闭塞开始后在绒毛尖端开始，随后在再灌注期间破坏周围组织。在肠系膜循环完全闭塞的早期形式中，净粘膜损伤的再灌注成分可能非常显著。(C)出版社：Academic Press
The significance of the reperfusion period in the pathophysiology of complete occlusion of the intestinal circulation is controversial. Our study was designed to evaluate the exact magnitude of reperfusion-induced intestinal mucosal damage in a standardized rat model of complete segmental arterial ischemia as a function of the occlusion time. Intestinal ischemia was maintained for 15, 30, or 60 min, or ischemia was followed by a 30-min reperfusion period. Intraarterial India ink perfusion was applied to visualize the mucosal vascularity changes induced by ischemia or ischemia-reperfusion. The height of the distributing arterioles of the villi and the average mucosal thickness were recorded by an image analysis system, and the degree of mucosal damage was established on a semiquantitative 0 to 5 grade scale. Ischemia induced erythrocyte obstructions at the villus tip, a progressive decrease in carbon-filled arteriole height, and a concomitant 7, 23, or 35% reduction of the mucosal thickness, The percentage decrease in perfused arteriole height/percentage mucosal thickness reduction ratio was 2.2, 1.5, or 1 during the 15-, 30-, or 60-min ischemia, respectively. Extravasation of the carbon tracer was observed in the 60-min ischemia group, During reperfusion, the mucosal layer was reduced by 27, 38, or 57%, respectively, compared with the baseline values. The arteriole height reduction/mucosal thickness reduction ratio was 1:1 in all ischemic-reperfused groups. The degree of mucosal damage was significantly increased during reperfusion after the 15-min ischemia. Microvessel obstruction is initiated at the villus tip following the onset of arterial occlusion, with subsequent destruction of the surrounding tissues during reperfusion. The reperfusion component of the net mucosal damage may be very significant in early forms of complete occlusion of the mesenteric circulation. (C) 1995 Academic Press, Inc.