Application of dipolar coupling data to the refinement of the solution structure of the sarcin-ricin loop RNA.
Application of dipolar coupling data to the refinement of the solution structure of the sarcin-ricin loop RNA.
复制标题
应用偶极耦合数据来细化八菌素-蓖麻毒素环 RNA 的溶液结构。
DOI：
10.1023/a:1011214214552
复制
发表时间：
2001
影响因子：
2.7
通讯作者：
Moore,PB
中科院分区：
文献类型：
--
作者：
Warren,JJ;Moore,PB
Residual dipolar couplings can provide the long-range information that most NMR solution structures lack. The use of such data in protein structure determinations is now fairly routine, but even though these data should be much more useful for nucleic acids, their application to nucleic acid structure determination is still in its infancy. Here we present a method for producing accurate, dipolar-refined structures of nucleic acids that is more efficient than those used previously, and apply it to E73, a 29 nucleotide RNA that includes the sarcin-ricin loop from rat 28S rRNA. The results enable us to address the differences between the crystal structure of E73 and the solution structure proposed for it previously.
