Prevalence and genotype identification of Toxoplasma gondii in suburban rodents collected at waste disposal sites
Prevalence and genotype identification of Toxoplasma gondii in suburban rodents collected at waste disposal sites
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DOI：
10.1051/parasite/2019027
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发表时间：
2019-05-01
期刊：
影响因子：
2.9
通讯作者：
Buzan, Elena
中科院分区：
文献类型：
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作者：
Ivovic, Vladimir;Potusek, Sandra;Buzan, Elena
为了评估弓形虫感染的流行率在本地和啮齿类动物的环境污染的指标，我们分析了小哺乳动物的脑组织收集的法律的和非法的垃圾场在斯洛文尼亚和克罗地亚的伊斯特拉部分。共分析了鼠科5种136只动物：黑鼠（Rattus rattus）、家鼠（Mus musculus）、林鼠（Apodemus sylvaticus）、黑线姬鼠（Apodemus agrarius）和黄颈姬鼠（Apodemus flavicollis）。采用定量聚合酶链反应（qPCR），T.除黑鼠外，其它动物脑组织匀浆中均检出弓形虫DNA，检出率为2.94%。其中，家鼠（Mus musculus）和林鼠（Apodemus sylvaticus）两个样本具有足够的DNA用于T.弓形虫分离株，其中我们证明了克隆的存在下，使用RFLP PCR与四个标记（SAG1，SAG2，GRA6和GRA7）。四分之三的感染动物（75%）是在垃圾场收集的。
To assess the prevalence of Toxoplasma gondii infection in native and commensal rodents as indicators of environmental pollution, we analyzed brain tissue from small mammals collected on legal and illegal waste sites in the Slovenian and Croatian parts of Istria. A total of 136 animals and five species of the family Muridae were analyzed: black rat (Rattus rattus), domestic mouse (Mus musculus), wood mouse (Apodemus sylvaticus), striped field mouse (Apodemus agrarius), and yellow-necked mouse (Apodemus flavicollis). Using quantitative Polymerase Chain Reaction (qPCR), T. gondii DNA was detected in four homogenized brain tissue samples (2.94%), from all of the analyzed species, except black rat. Out of these, two samples, domestic mouse (Mus musculus) and wood mouse (Apodemus sylvaticus) had sufficient DNA for genotyping of T. gondii isolates in which we demonstrated the presence of clonal type II using RFLP PCR with four markers (SAG1, SAG2, GRA6 and GRA7). Three of four infected animals (75%) were collected on dumpsites.