Protection of mouse bone marrow by Si-Wu-Tang against whole body irradiation
Protection of mouse bone marrow by Si-Wu-Tang against whole body irradiation
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DOI：
10.1016/0378-8741(96)01400-6
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发表时间：
1996-06-01
影响因子：
5.4
通讯作者：
Lin, CC
中科院分区：
文献类型：
--
作者：
Hsu, HY;Ho, YH;Lin, CC
采用造血干细胞集落形成试验（脾集落形成单位存活率）和造血功能试验（包括血象和红细胞压积的变化）评价四物汤对辐射后骨髓损伤的消除作用。在1 ~ 9戈伊的剂量范围内，绘制了单纯放疗和四物汤联合放疗的细胞存活曲线和剂量-反应曲线。照射前7天连续分次给予四物汤20 mg/20 g体重。四物汤组干细胞的辐射抗性高于单纯辐射组。但白细胞的抗辐射作用不如红细胞、血小板和红细胞压积显著。提示四物汤的抗辐射作用具有剂量依赖性，在4戈伊照射后，四物汤的抗辐射作用减弱。四物汤给药后，周转期短的血象（如白细胞）可能受影响较小。
The ability of Si-Wu-Tang to eliminate bone marrow damage after radiation was assessed using both a clonogenic assay (survival of colony forming units spleen - CFUs) of stem cell survival and a hematopoietic functional assay including the changes of hemograms and hematocrit. Cell survival curves and dose-response curves for radiation alone and Si-Wu-Tang administration with radiation were constructed over the dose range of 1 to 9 Gy. Si-Wu-Tang was given 7 days before irradiation at a consecutive fractionated dose of 20 mg/20 g body weight. The radioresistance of stem cells treated by Si-Wu-Tang was higher than that of those treated by irradiation alone. However, the antiradiation effect of leukocytes was not as significant as that of erythrocytes, thrombocytes and hematocrit. These data suggest that the anti-radiation effect by Si-Wu-Tang is dependent on dose and will be less after 4 Gy irradiation. Hemograms with a short turnover period, such as leukocytes, may be less affected after Si-Wu-Tang administration.