Physical characterization and in vitro evaluation of 3D printed hydroxyapatite, tricalcium phosphate, zirconia, alumina, and SiAlON structures made by lithographic ceramic manufacturing
Physical characterization and in vitro evaluation of 3D printed hydroxyapatite, tricalcium phosphate, zirconia, alumina, and SiAlON structures made by lithographic ceramic manufacturing
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DOI：
10.1557/adv.2020.229
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发表时间：
2020-04
期刊：
影响因子：
0.8
通讯作者：
Alexander K. Nguyen;P. Goering;Shelby A. Skoog;R. Narayan
中科院分区：
文献类型：
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作者：
Alexander K. Nguyen;P. Goering;Shelby A. Skoog;R. Narayan
在这项研究中，平版陶瓷制造用于创建固体芯片的羟基磷灰石，磷酸三钙，氧化锆，氧化铝，和SiAlON陶瓷。每种材料的X射线粉末衍射证实，碎片是结晶的，几乎没有可能由剩余的聚合物粘合剂产生的无定形特征，并且完全由陶瓷原料组成。表面形貌和粗糙度，其特征在于使用原子力显微镜。在每种材料类型上与成骨补充剂一起培养的人骨髓干细胞表达碱性磷酸酶水平，这是成骨分化的早期标志物，与在玻璃对照上培养的细胞相当。然而，在含磷酸三钙的材料上培养的细胞表达较低水平的ALP，这表明在这种材料上骨诱导受损。应对这些材料进行进一步分析，以确定材料和分析方法结合的潜在问题。
In this study, lithographic ceramic manufacturing was used to create solid chips out of hydroxyapatite, tricalcium phosphate, zirconia, alumina, and SiAlON ceramic. X-ray powder diffraction of each material confirmed that the chips were crystalline, with little amorphous character that could result from remaining polymeric binder, and were composed entirely out of the ceramic feedstock. Surface morphologies and roughnesses were characterized using atomic force microscopy. Human bone marrow stem cells cultured with osteogenic supplements on each material type expressed alkaline phosphatase levels, an early marker of osteogenic differentiation, on par with cells cultured on a glass control. However, cells cultured on the tricalcium phosphate-containing material expressed lower levels of ALP suggesting that osteoinduction was impaired on this material. Further analyses should be conducted with these materials to identify underlying issues of the combination of material and analysis method.