Desiccation tolerance in Staphylococcus aureus
Desiccation tolerance in Staphylococcus aureus
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DOI：
10.1007/s00203-010-0653-x
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发表时间：
2011-02-01
影响因子：
2.8
通讯作者：
Foster, Simon J.
中科院分区：
文献类型：
--
作者：
Chaibenjawong, Plykaeow;Foster, Simon J.
金黄色葡萄球菌是一种耐多药病原体，不仅会导致多种人类疾病，而且能够在潜在的干燥和压力环境中生存，如人的鼻子，皮肤和无生命的表面，如衣服和表面。这项研究调查了控制金黄色葡萄球菌脱水耐受性的参数，并确定了参与这一过程的几个成分。初步确定了温度、生长阶段、细胞密度、干燥时间和保护剂等环境参数在耐脱水中的作用。这建立了一个强大的干燥耐受性模型，在该模型中，金黄色葡萄球菌能够在干燥的塑料表面上存活超过1097天。利用随机筛选和定义的突变体相结合的方法，鉴定了clpX、SIGB和yjbH是耐脱水所必需的。ClpX是依赖于ATP的ClpXP蛋白水解酶的一部分，对蛋白质的周转很重要，而YjbH具有拟议的连锁功能。SIGB是一种辅助西格玛因子，在广泛的应力抵抗中发挥作用。了解控制脱水耐受性的分子机制可能确定可以利用的临界点，以防止这种危险病原体在医院和社区传播。
Staphylococcus aureus is a multidrug-resistant pathogen that not only causes a diverse array of human diseases, but also is able to survive in potentially dry and stressful environments, such as the human nose, on skin and on inanimate surfaces such as clothing and surfaces. This study investigated parameters governing desiccation tolerance of S. aureus and identified several components involved in the process. Initially, the role of environmental parameters such as temperature, growth phase, cell density, desiccation time and protectants in desiccation tolerance were determined. This established a robust model of desiccation tolerance in which S. aureus has the ability to survive on dry plastic surfaces for more than 1,097 days. Using a combination of a random screen and defined mutants, clpX, sigB and yjbH were identified as being required for desiccation tolerance. ClpX is a part of the ATP-dependent ClpXP protease, important for protein turnover, and YjbH has a proposed linked function. SigB is an accessory sigma factor with a role in generalized stress resistance. Understanding the molecular mechanisms that govern desiccation tolerance may determine the break points to be exploited to prevent the spread of this dangerous pathogen in hospitals and communities.