A LC-MS-based workflow for measurement of branched fatty acid esters of hydroxy fatty acids.
A LC-MS-based workflow for measurement of branched fatty acid esters of hydroxy fatty acids.
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DOI：
10.1038/nprot.2016.040
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发表时间：
2016-04
期刊：
影响因子：
14.8
通讯作者：
Saghatelian A
中科院分区：
文献类型：
--
作者：
Zhang T;Chen S;Syed I;Ståhlman M;Kolar MJ;Homan EA;Chu Q;Smith U;Borén J;Kahn BB;Saghatelian A
羟基脂肪酸的支链脂肪酸酯（FAHFA）是最近发现的一类具有抗糖尿病和抗炎作用的内源性哺乳动物脂质。我们鉴定了16个不同的FAHFA家族，例如羟基硬脂酸的支链棕榈酸酯（PAHSA），并且每个家族由多个异构体组成，其中支链酯在不同位置（例如5-和9-PAHSA）。我们预计PAHSA测量作为代谢和炎症性疾病的标志物的需求增加。在本方案中，我们提供了从人或小鼠组织中提取FAHFA和随后进行液相色谱-质谱（LC-MS）的详细描述。对于6-12的样本量，时间范围为2-3天。
Branched Fatty Acid esters of Hydroxy Fatty Acids (FAHFAs) are a recently discovered class of endogenous mammalian lipids with anti-diabetic and anti-inflammatory effects. We identified 16 different FAHFA families, such as branched Palmitic Acid esters of Hydroxy Stearic Acids (PAHSA), and each family consists of multiple isomers where the branched ester is at different positions (e.g. 5- and 9-PAHSA). We anticipate increased need for PAHSA measurements as markers of metabolic and inflammatory diseases. In this protocol, we provide a detailed description of the extraction and subsequent liquid chromatography–mass spectrometry (LC–MS) of FAHFAs from human or mouse tissues. For a sample size of 6–12 the time frame is 2–3 days.