RESTRICTION ENDONUCLEASE ANALYSIS OF MITOCHONDRIAL-DNA FROM NORMAL AND TEXAS CYTOPLASMIC MALE-STERILE MAIZE

RESTRICTION ENDONUCLEASE ANALYSIS OF MITOCHONDRIAL-DNA FROM NORMAL AND TEXAS CYTOPLASMIC MALE-STERILE MAIZE
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DOI:
10.1126/science.193.4248.158
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发表时间:
1976-01-01
期刊:
影响因子:
56.9
通讯作者:
PRING, DR
PRING, DR
中科院分区:
综合性期刊1区
文献类型:
--
作者:
LEVINGS, CS;PRING, DR

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用来自流感嗜血杆菌(Hemophilusinfluenzae)的大肠杆菌(EscherichiacoliordIII)的限制性内切酶RI消化正常玉米和细胞质雄性不育玉米的线粒体DNA。所得片段的电泳显示两种细胞质类型之间的区别。这些区别表明,负责细胞质雄性不育的因素位于线粒体DNA中,线粒体基因组不是父系遗传的。
Mitochondrial DNA from normal and cytoplasmic male-sterile maize was digested with restriction endonucleases RI fromEscherichia colior dIII fromHemophilus influenzae. Electrophoresis of resulting fragments revealed distinctions between the two cytoplasmic types. These distinctions suggest that factors responsible for cytoplasmic male sterility are located in the mitochondrial DNA, and that the mitochondrial genome is not inherited paternally.