Sequence and Regional Distribution of the mRNA Encoding the α2 Polypeptide of Rat γ‐Aminobutyric AcidA Receptors
Sequence and Regional Distribution of the mRNA Encoding the α2 Polypeptide of Rat γ‐Aminobutyric AcidA Receptors
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大鼠γ-氨基丁酸A受体α2多肽mRNA序列及区域分布
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
A. Tobin
中科院分区：
文献类型：
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作者：
M. Khrestchatisky;A. MacLennan;N. Tillakaratne;M. Chiang;A. Tobin
摘要：大鼠海马区c DNA文库编码α-氨基丁酸/苯二氮(BZ)受体γ多肽的异构体。其推导的氨基酸序列与牛GABA受体α-2多肽的氨基酸序列同源性为96%。该多肽具有所有已报道的GAAA受体α多肽的共同特征，并与先前描述的大鼠α多肽有71-76%的同源性。大部分差异在于推测的胞外和胞内结构域。在Northern blotts上，α2cDNA检测到两个mRNAs，这两个mRNAs发现于皮质、海马体和纹状体，这些脑区富含药理学上定义的“BZ II型”受体。其他研究人员此前已经证明，GABA受体的α多肽在很大程度上决定了重组受体的BZ结合特性。α-2mRNAs在大鼠脑内的分布表明，α-2亚基可能确实参与了BZ-II型受体。
Abstract: A cDNA from a rat hippocampal cDNA library encodes an isoform of the α polypeptide of the γ‐aminobutyric acid (GABA)/benzodiazepine (BZ) receptor. Its deduced amino acid sequence is 96% identical to that of the α2 polypeptide of the bovine GABAA receptor. The polypeptide has features shared by all previously reported GABAA receptor α polypeptides and shares 71–76% identity with previously described rat α polypeptides. Most of the differences lie in the presumed extracellular and intracellular domains. On Northern blots, the α2 cDNA detects two mRNAs, which are found in cortex, hippocampus, and striatum, brain regions enriched in pharmacologically defined “BZ type II” receptors. Other workers have previously shown that the α polypeptides of the GABAA receptor largely determine the BZ binding properties of reconstituted receptors. The distribution of α2 mRNAs in rat brain suggests that the α2 subunit may indeed be involved in the BZ type II receptors.