Intracellular replication of Edwardsiella ictaluri in channel catfish macrophages
Intracellular replication of Edwardsiella ictaluri in channel catfish macrophages
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DOI：
10.1577/h05-025.1
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发表时间：
2006-06-01
影响因子：
1.2
通讯作者：
Thune, Ronald L.
中科院分区：
文献类型：
--
作者：
Booth, Natha J.;Elkamel, Ahmad;Thune, Ronald L.
体外研究了鮰鱼爱德华氏菌进入斑点叉尾鮰头肾来源的巨噬细胞、存活和复制的能力。将原代巨噬细胞培养物暴露于鲫鱼中，并在 0（感染后 90 分钟）、4、8 和 12 小时后取样。数据表明，孵化 4、8 和 12 小时后，鮰鱼 E. ictaluri 的细菌数量分别增加了 2.6、5.1 和 7.1 倍。对受感染细胞培养物的显微镜检查显示，透明、宽敞的液泡中含有大量细胞内细菌。与同一时间点用热灭活血清或无血清预处理相比，用正常血清调理导致鲫鱼在0小时内的内化显着增加，并且用正常血清预处理的细菌在4和8小时后保持显着高于其他处理的细菌数量。然而，调理作用并不影响鲫鱼进入细胞后的复制能力，因为无论血清预处理如何，在所有时间点细菌数量都有统计学上显着的增加。
The ability of Edwardsiella ictaluri to enter, survive, and replicate in head kidney-derived macrophages from channel catfish Ictalurus punctatus was investigated in vitro. Primary macrophage cultures were exposed to E. ictaluri and were sampled after 0 (90 min postinfection), 4, 8, and 12 h. The data indicated that bacterial numbers for E. ictaluri increased 2.6-, 5.1-, and 7.1-fold after 4, 8, and 12 h of incubation, respectively. Microscopic examination of infected cell cultures revealed numerous intracellular bacteria that were contained within clear, spacious vacuoles. Opsonization with normal serum resulted in significantly greater internalization of E. ictaluri at 0 h compared with pretreatment with heat-inactivated serum or no serum at the same time point, and bacteria pretreated with normal serum maintained significantly greater numbers than those in the other treatments after 4 and 8 h. Opsonization, however, did not affect the ability of E. ictaluri to replicate once the cell was entered, as there were statistically significant increases in bacterial numbers at all time points regardless of the serum pretreatment.