The Examination of the Coloring Mechanism of the Human Esophageal Epithelium by Lugol's Solution
The Examination of the Coloring Mechanism of the Human Esophageal Epithelium by Lugol's Solution
复制标题
卢戈氏溶液检查人食管上皮的着色机制
DOI：
10.2468/jbes.40.252
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
M. Fujimaki
中科院分区：
文献类型：
--
作者：
M. Sawataishi;Y. Karaki;M. Kawaguchi;M. Saitoh;T. Saeki;A. Yamada;K. Shimazaki;S. Munakata;T. Sakamoto;T. Shinbo;K. Tazawa;M. Fujimaki
众所周知，正常食管上皮是用改良的卢戈氏溶液染色的。另一方面，通过内窥镜染色法，卢戈氏溶液不会对食道的发育不良或癌性粘膜进行染色。使用卢戈氏溶液使这些食管粘膜着色或不着色的机制尚不清楚。因此，为了阐明这一机制，我们使用光学显微镜检查了用卢戈氏溶液手术切除的食管组织切片。同时，我们利用X射线微量分析技术在每个滴注了卢戈溶液的组织中检测到了碘元素。光镜观察发现，正常食管粘膜的角质层和颗粒层细胞被卢戈氏溶液强烈染色。发育不良上皮在其表层表现出轻微的着色特性。卢戈氏溶液没有对癌性病变进行组织学染色。在X射线微量分析研究中，角质层和颗粒层细胞显示出碘的高峰。在发育不良粘膜的浅表区域检测到碘的中间峰。在癌症组织的任何其他部分均未发现碘峰。这些结果表明，正常食管上皮、发育异常区域和癌区域的着色特性的差异对应于这些区域的这些细胞中的每一个与碘的亲和力。
It has been well known that the normal esophageal epithelium is stained by a modified Lugol's solution. On the other hand, displastic or cancerous mucosa of the esophagus is not stained by Lugol's solution through the endoscopic staining method. It is not clear what kind of mechanism causes the coloring or uncoloring of these esophageal mucosa using Lugol's solution. Therefore, to clarify this mechanism, we examined the sections of surgically resected esophageal tissues with Lugol's solution using the light microscopy. At the same time, we detected an iodine element in each tissue instilled with Lugol's solution by means of X-ray microanalytic technique. In light microscopic study, the cells of the stratum corneum and stratum granulosa of the normal esophageal mucosa were strongly stained by Lugol's solution. Dysplastic epithelium showed a slight coloration property in its superficial layer. No cancerous lesions were histologically stained by Lugol's solution. In X-ray microanalytic study, the cells of stratum corneum and stratum granulosa displayed a high peak for iodine. An intermediate peak for iodine was detected in the superficial zone of the displastic mucosa. No peaks for iodine were found in any other portion of the cancer tissues. These results suggested that the difference in the coloration property of the normal esophageal epithelium, dysplastic regions and cancerous regions corresponded to the affinity of each of these cells of these regions with iodine.