Potentiation of the in vitro Antistaphylococcal Effect of Oxacillin and Tetracycline by the Anti-Inflammatory Drug Diacetyl Rhein
Potentiation of the in vitro Antistaphylococcal Effect of Oxacillin and Tetracycline by the Anti-Inflammatory Drug Diacetyl Rhein
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DOI：
10.1159/000363730
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发表时间：
2014-08
期刊：
影响因子：
3.3
通讯作者：
Samnang Nguon;P. Nový;L. Kokoska
中科院分区：
文献类型：
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作者：
Samnang Nguon;P. Nový;L. Kokoska
背景资料：抗炎药双乙酰大黄酸在我们以前的研究中被发现对各种耐药菌株具有良好的抗葡萄球菌作用。本文研究了双乙酰大黄酸与苯唑西林和四环素对13株标准菌株和临床分离的金黄色葡萄球菌（包括对红霉素、甲氧西林和四环素耐药的金黄色葡萄球菌）的体外联合作用。研究方法：采用微量肉汤稀释法测定最低抑菌浓度，并根据部分抑菌浓度之和（MIC）评价组合的效果。结果：观察到对所有S.金黄色葡萄球菌菌株的生长抑制率为0.258-1，而二乙酰大黄酸-苯唑西林似乎是最有效的组合，协同抑制4种测试菌株的生长。结论：据我们所知，这是第一次报道双乙酰大黄酸的协同抗菌作用。我们的研究结果表明，这种有前途的化合物，其协同抗感染的潜力，作为一种联合抗炎和协同抗菌作用的代理进一步评估。
Background: The anti-inflammatory drug diacetyl rhein has been found to possess promising antistaphylococcal effects against various drug-resistant strains in our previous study. In the present work, we explored the in vitro combinatory interactions of diacetyl rhein with oxacillin and tetracycline against 13 standard strains and clinical isolates of Staphylococcus aureus, including those resistant to erythromycin, methicillin and tetracycline. Methods: Minimum inhibitory concentrations were determined by broth microdilution assay, and the effects of combinations were evaluated according to the sum of fractional inhibitory concentrations (ΣFICs). Results: Synergistic or additive effects were observed against all S. aureus strains (ΣFIC 0.258-1), whereas diacetyl rhein-oxacillin appeared to be the most effective combination, synergistically inhibiting the growth of 4 strains tested. Conclusion: To our best knowledge, this is the first report on a synergistic antibacterial effect of diacetyl rhein. Our results suggest this promising compound for further evaluation of its synergistic anti-infective potential as an agent with a combined anti-inflammatory and synergistic antibacterial action.