Visualization of cortical, subcortical and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically implanted lenses.
Visualization of cortical, subcortical and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically implanted lenses.
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DOI：
10.1038/nprot.2016.021
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发表时间：
2016-03
期刊：
影响因子：
14.8
通讯作者：
Stuber GD
中科院分区：
文献类型：
--
作者：
Resendez SL;Jennings JH;Ung RL;Namboodiri VM;Zhou ZC;Otis JM;Nomura H;McHenry JA;Kosyk O;Stuber GD
用于可视化动态细胞活动的遗传编码钙指标极大地扩展了我们对大脑的了解。然而，由于大脑的光散射特性以及传统成像技术的尺寸和刚性，在头部固定行为任务期间，体内钙成像已被限制于浅表大脑结构。这种限制现在可以通过利用微型集成显微镜结合可植入的显微内窥镜透镜来引导光进出大脑来规避，从而允许在自然行为期间光学访问脑深部（或浅表）神经系综。在这里，我们描述的程序步骤进行这样的成像研究使用小鼠。然而，我们预计该协议可以很容易地适用于其他小型脊椎动物。该方案的成功完成将允许神经元活动的细胞成像和具有足够统计能力的数据集的生成，以将神经活动与刺激呈现、生理状态和复杂行为任务的其他方面相关联。该方案需要6-11周才能完成。
Genetically encoded calcium indicators for visualizing dynamic cellular activity have greatly expanded our understanding of the brain. However, due to light scattering properties of the brain as well as the size and rigidity of traditional imaging technology, in vivo calcium imaging has been limited to superficial brain structures during head fixed behavioral tasks. This limitation can now be circumvented by utilizing miniature, integrated microscopes in conjunction with an implantable microendoscopic lens to guide light into and out of the brain, thus permitting optical access to deep brain (or superficial) neural ensembles during naturalistic behaviors. Here, we describe procedural steps to conduct such imaging studies using mice. However, we anticipate the protocol can be easily adapted for use in other small vertebrates. Successful completion of this protocol will permit cellular imaging of neuronal activity and the generation of data sets with sufficient statistical power to correlate neural activity with stimulus presentation, physiological state, and other aspects of complex behavioral tasks. This protocol takes 6–11 weeks to complete.