Regulation of soybean seed germination through ethylene production in response to reactive oxygen species
Regulation of soybean seed germination through ethylene production in response to reactive oxygen species
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DOI：
10.1093/aob/mcs240
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发表时间：
2013-01-01
期刊：
影响因子：
4.2
通讯作者：
Iwaya-Inoue, Mari
中科院分区：
文献类型：
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作者：
Ishibashi, Yushi;Koda, Yuka;Iwaya-Inoue, Mari
尽管具有毒性，但活性氧 (ROS) 在植物细胞信号传导途径中发挥着重要作用，例如介导对应激或感染的反应以及较低水平的程序性细胞死亡。尽管研究表明过氧化氢（H2O2）可以促进拟南芥、大麦、小麦、水稻和向日葵等多种植物的种子发芽，但H2O2在大豆种子发芽中的作用尚不清楚。因此，本研究旨在探讨ROS、植物激素与大豆种子萌发之间的关系。利用抗氧化剂N-乙酰半胱氨酸对大豆种子的萌发情况、乙烯生物合成相关基因的表达量、内源乙烯含量以及根尖细胞的数量和面积进行检测，以抵消ROS的影响。H2O2促进发芽，N-乙酰半胱氨酸抑制发芽，表明ROS参与了大豆发芽的调控。吸胀后在胚轴中产生H2O2。 N-乙酰半胱氨酸抑制与乙烯生物合成和内源乙烯产生相关的基因的表达。有趣的是，乙烯利（可转化为乙烯）和 H2O2 逆转了 N-乙酰半胱氨酸对种子发芽的抑制。此外，形态学分析显示，N-乙酰半胱氨酸抑制根尖细胞伸长，这种抑制也可以通过乙烯利或H2O2处理逆转，就像发芽时的情况一样。在大豆种子中，吸胀后胚轴产生的ROS诱导内源乙烯的产生，从而促进根尖细胞伸长。这似乎就是 ROS 调节大豆种子发芽的方式。
Despite their toxicity, reactive oxygen species (ROS) play important roles in plant cell signalling pathways, such as mediating responses to stress or infection and in programmed cell death, at lower levels. Although studies have indicated that hydrogen peroxide (H2O2) promotes seed germination of several plants such as Arabidopsis, barley, wheat, rice and sunflower, the role of H2O2 in soybean seed germination is not well known. The aim of this study therefore was to investigate the relationships between ROS, plant hormones and soybean seed germination.An examination was made of soybean seed germination, the expression of genes related to ethylene biosynthesis, endogenous ethylene contents, and the number and area of cells in the root tip, using N-acetylcysteine, an antioxidant, to counteract the effect of ROS.H2O2 promoted germination, which N-acetylcysteine suppressed, suggesting that ROS are involved in the regulation of soybean germination. H2O2 was produced in the embryonic axis after imbibition. N-Acetylcysteine suppressed the expression of genes related to ethylene biosynthesis and the production of endogenous ethylene. Interestingly, ethephon, which is converted to ethylene, and H2O2 reversed the suppression of seed germination by N-acetylcysteine. Furthermore, morphological analysis revealed that N-acetylcysteine suppressed cell elongation at the root tip, and this suppression was also reversed by ethephon or H2O2 treatments, as was the case in germination.In soybean seeds, ROS produced in the embryonic axis after imbibition induce the production of endogenous ethylene, which promotes cell elongation in the root tip. This appears to be how ROS regulate soybean seed germination.