Studies on the glycosidases in jack bean meal. II. Sepation of various glycosidases by isoelectric focusing.
Studies on the glycosidases in jack bean meal. II. Sepation of various glycosidases by isoelectric focusing.
复制标题
刀豆粕中糖苷酶的研究.
DOI：
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发表时间：
1968
影响因子：
4.8
通讯作者：
S. Li
中科院分区：
文献类型：
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作者：
Y. Li;S. Li
摘要采用等电聚焦技术从刀豆粕中同时分离β-N-乙酰氨基葡萄糖苷酶、α-甘露糖苷酶和β-半乳糖苷酶。这些糖苷酶被分离和纯化80- 170倍，产率为40 - 60%。发现这些酶的pI值分别为4.8、6.2和8.4。
Abstract β-N-Acetylglucosaminidase, α-mannosidase, and β-galactosidase were simultaneously separated from jack bean meal extract by means of isoelectric focusing technique. These glycosidases were isolated and purified 80- to 170-fold with yields of 40 to 60%. pI values of these enzymes were found to be 4.8, 6.2, and 8.4, respectively.