Morphological and molecular identification of Sarcocystis spp. from the sika deer (Cervus nippon), including two new species Sarcocystis frondea and Sarcocystis nipponi
Morphological and molecular identification of Sarcocystis spp. from the sika deer (Cervus nippon), including two new species Sarcocystis frondea and Sarcocystis nipponi
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DOI：
10.1007/s00436-018-5816-8
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发表时间：
2018-02
影响因子：
2
通讯作者：
E. Rudaitytė-Lukošienė;P. Prakas;D. Butkauskas;L. Kutkienė;I. Vepštaitė-Monstavičė;E. Servienė
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文献类型：
--
作者：
E. Rudaitytė-Lukošienė;P. Prakas;D. Butkauskas;L. Kutkienė;I. Vepštaitė-Monstavičė;E. Servienė
对25只立陶宛饲养的西卡鹿（Cervus nippon）的膈肌进行了肉孢子虫（Sarcocystis）检查。应用光学显微镜和透射电子显微镜对两个肉孢子虫新种--弗氏肉孢子虫（Sarcocystis frondea）和日本肉孢子虫（Sarcocystis nipponi）进行了观察，并对其进行了18 S rDNA和细胞色素c氧化酶I亚基（cox 1）序列分析。光镜下，弗氏棘球绦虫和日本棘球绦虫的包囊呈带状，囊壁有指状突起。透射电镜下，羊栖菜突起约9 × 1-1.5 μm，充满清晰可见的电子致密物质和微管，呈39型。而日本沼虾的圆顶状突起（约9 × 0.2 μm）充满了微管，微管延伸到基质层，呈9 o型。此外，三个已知的肉孢子虫属，在西卡中发现了以前在其它鹿科动物中作为中间宿主的中间宿主的圆胸肉孢子虫、卵形肉孢子虫和截形肉孢子虫。18 S rDNA比Cox 1更适合于划分近缘的鹿科肉孢子虫种。系统发育结果表明，食腐鸟类可能是S. frondea的最终宿主。根据对西卡鹿体内住肉孢子虫形态学资料的总结，这种宿主至少应住有9种不同的住肉孢子虫。
Diaphragm muscles of 25 sika deer (Cervus nippon) farmed in Lithuania were examined for sarcocysts ofSarcocystisspecies. Two newSarcocystisspecies,Sarcocystis frondeaandSarcocystis nipponi, were observed using light microscopy (LM) and transmission electron microscopy (TEM) and characterized by 18S ribosomal DNA (rDNA) and subunit I of cytochrome c oxidase (cox1) sequence analyses. By LM, sarcocysts ofS.frondeaandS.nipponiwere ribbon-shaped and had finger-like sarcocyst wall protrusions, respectively. Under TEM, protrusions ofS.frondeawere about 9 × 1–1.5 μm, filled with clearly visible electron-dense substance and microtubules, type 39-like. Whereas, protrusions (about 9 × 0.2 μm) ofS.nipponiarose from dome-shaped bases were filled with microtubules extending to the ground substance layer, type 9o-like. Moreover, three knownSarcocystisspp.,Sarcocystis entzerothi,Sarcocystis ovalis, andSarcocystis truncatapreviously described in other cervids as intermediate hosts, were characterized in sika deer. Thecox1was more suitable than 18S rDNA delimitating closely relatedSarcocystisspecies from cervids. The phylogenetic results suggest that scavenger birds could be definitive hosts ofS.frondea. According to the summarized morphological data onSarcocystisfound in the sika deer, such host should harbor at least nine differentSarcocystisspecies.