Screening plateletpheresis donors for HLA antibodies on two high-throughput platforms and correlation with recipient outcome.
Screening plateletpheresis donors for HLA antibodies on two high-throughput platforms and correlation with recipient outcome.
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DOI：
10.1111/j.1537-2995.2010.02874.x
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发表时间：
2011-03
期刊：
影响因子：
2.9
通讯作者：
Stroncek DF
中科院分区：
文献类型：
--
作者：
Quillen K;Medrano C;Adams S;Peterson B;Hackett J;Leitman SF;Klein HG;Stroncek DF
为了确定输注含有白细胞抗体的血浆的流行率和影响，我们比较了两种高通量 HLA 抗体筛查试验，并前瞻性地检查了所有血小板接受者的医疗记录，以检测 TRALI 和其他输血反应的细微表现。使用微珠 (Labscreen PRA) 和微芯片 (Dynachip) 测定法对 136 名血小板去除捐献者的血清样本进行了 HLA I 类和 II 类抗体检测。在输血前后，对所有接受者的电子病历进行了生命体征和护理文件的审查。在截断值为 0.25 的微芯片检测中，2.9% 的样本呈 I 类抗体阳性，8.9% 的样本呈 II 类抗体阳性；以 0.1 为截止值，结果分别为 14.9% 和 21.6%。在微珠测定（NBG 比率 1.5）中，15% 的 I 类抗体呈阳性，21% 的 II 类抗体呈阳性。在没有妊娠或输血史的捐献者中，HLA 抗体的患病率为 17%，在有此类历史的捐献者中，HLA 抗体的患病率为 47%。共向 67 名患者进行了 265 次血小板输注。没有报告反应；然而，在 7 次输血事件中发现了症状或生命体征变化。抗体阳性单位的反应发生率为2.7% (2/75)，抗体阴性单位的反应发生率为2.6% (5/190)。微珠和微芯片测定产生了相似的结果。在有妊娠或输血史的捐献者中，HLA 抗体的患病率较高，但在接受来自具有 HLA 抗体的捐献者的成分的受者中，输血反应的发生率并未增加。
To determine the prevalence and impact of transfusing plasma containing leukocyte antibodies, we compared two high-throughput HLA antibody screening assays, and prospectively examined the medical records of all platelet recipients to detect subtle manifestations of TRALI and other transfusion reactions. Serum samples from 136 plateletpheresis donors were tested for HLA Class I and II antibodies using microbead (Labscreen PRA) and microchip (Dynachip) assays. Electronic medical records of all recipients were reviewed for vital signs and nursing documentation before and after transfusion. In the microchip assay with a cutoff value of 0.25, 2.9% of samples were positive for Class I, and 8.9% for Class II antibodies; with a cutoff value of 0.1, the results were 14.9% and 21.6% respectively. In the microbead assay (NBG ratio 1.5), 15% were positive for Class I, and 21% for Class II antibodies. The prevalence of HLA antibodies was 17% in donors without pregnancy or transfusion history, and 47% in donors with such history. The platelets were transfused in 265 episodes to 67 patients. There were no reported reactions; however, symptoms or vital sign changes were noted in 7 transfusion episodes. The incidence of reactions was 2.7% (2/75) for antibody-positive units and 2.6% (5/190) for antibody-negative units. Microbead and microchip assays yielded similar results. The prevalence of HLA antibodies was greater in donors with a history of pregnancy or transfusion, but no increase in the incidence of transfusion reactions was noted in recipients of components from donors with HLA antibodies.
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影响因子：
2.7
作者：
Fadeyi, E. A.;Adams, S.;Stroncek, D. F.
通讯作者：
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影响因子：
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作者：
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通讯作者：
Funk, M. B.
影响因子：
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作者：
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Ambruso, DR