The Generation and Application of Patient-Derived Xenograft Model for Cancer Research.
The Generation and Application of Patient-Derived Xenograft Model for Cancer Research.
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患者衍生的异种移植模型在癌症研究中的产生和应用。
DOI：
10.4143/crt.2017.307
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发表时间：
2018-01
影响因子：
4.6
通讯作者：
Chang S
中科院分区：
文献类型：
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作者：
Jung J;Seol HS;Chang S
建立适当的临床前模型对于转化癌症研究至关重要。迄今为止最常见的方法是移植来自患者的癌细胞系。虽然这种异种移植模型很容易生成，但有一些局限性，因为这种癌症模型不能充分代表每个癌症患者的独特特征。此外，越来越多的证据表明癌症是一种高度异质性的疾病，因此肿瘤由具有不同特征的癌细胞组成。为了避免异种移植模型和患者肿瘤之间的这些差异，患者来源的异种移植（PDX）模型已被积极生成和应用。 PDX模型可以通过将患者肿瘤的癌组织直接植入免疫缺陷小鼠体内来开发，从而保留细胞间相互作用和肿瘤微环境。此外，PDX模型作为临床前模型在药物筛选、生物标志物开发和临床联合试验中显示出优势。在这篇综述中，我们将详细总结 PDX 的方法和应用，并涵盖开发该模型用于临床前研究的关键问题。
Establishing an appropriate preclinical model is crucial for translational cancer research. The most common way that has been adopted by far is grafting cancer cell lines, derived from patients. Although this xenograft model is easy to generate, but has several limitations because this cancer model could not represent the unique features of each cancer patient sufficiently. Moreover, accumulating evidences demonstrate cancer is a highly heterogeneous disease so that a tumor is comprised of cancer cells with diverse characteristics. In attempt to avoid these discrepancies between xenograft model and patients’ tumor, a patient-derived xenograft (PDX) model has been actively generated and applied. The PDX model can be developed by the implantation of cancerous tissue from a patient’s tumor into an immune-deficient mouse directly, thereby it preserves both cell-cell interactions and tumor microenvironment. In addition, the PDX model has shown advantages as a preclinical model in drug screening, biomarker development and co-clinical trial. In this review, we will summarize the methodology and applications of PDX in detail, and cover critical issues for the development of this model for preclinical research.
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影响因子：
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作者：
Gonçalves A;Bertucci F;Guille A;Garnier S;Adelaide J;Carbuccia N;Cabaud O;Finetti P;Brunelle S;Piana G;Tomassin-Piana J;Paciencia M;Lambaudie E;Popovici C;Sabatier R;Tarpin C;Provansal M;Extra JM;Eisinger F;Sobol H;Viens P;Lopez M;Ginestier C;Charafe-Jauffret E;Chaffanet M;Birnbaum D
通讯作者：
Birnbaum D
通过白血病精确医学方法的微调患者衍生的异种移植模型。
DOI：
10.1136/jim-2016-000076
发表时间：
2016-03
期刊：
Journal of investigative medicine : the official publication of the American Federation for Clinical Research
影响因子：
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作者：
Francis OL;Milford TA;Beldiman C;Payne KJ
通讯作者：
Payne KJ
影响因子：
4.6
作者：
Aytes, Alvaro;Mollevi, David G.;Villanueva, Alberto
通讯作者：
Villanueva, Alberto
影响因子：
8.8
作者：
Garrido-Laguna, I.;Tan, A. C.;Hidalgo, M.
通讯作者：
Hidalgo, M.
影响因子：
64.8
作者：
通讯作者：
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