Dazl Promotes Germ Cell Differentiation from Embryonic Stem Cells
Dazl Promotes Germ Cell Differentiation from Embryonic Stem Cells
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Dazl 促进胚胎干细胞分化为生殖细胞
DOI：
10.1093/jmcb/mjp026
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发表时间：
2009-12-01
影响因子：
5.5
通讯作者：
Feng, Lixin
中科院分区：
文献类型：
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作者：
Yu, Zhuo;Ji, Ping;Feng, Lixin
胚胎干细胞（ES）可以通过形成胚状体（EBs）而获得生殖细胞。在这里，我们描述了一种转基因表达方法，直接从体外胚胎干细胞中获得生殖细胞，而不形成EB。通过异位表达一种生殖细胞特异性rna结合蛋白Deleted in Azoospermia-Like (Dazl)，从培养的小鼠ES （mES）细胞中诱导出可运动的尾精子和卵母细胞。在mES细胞中，短暂过表达Dazl抑制了Nanog的表达，但诱导了生殖细胞核抗原的表达。Dazl敲低导致Stella、MVH和Prdm1等生殖细胞标志物的表达减少。我们的研究表明Dazl是控制生殖细胞分化的主控基因，Dazl的异位表达促进了小鼠胚胎干细胞在体外向配子的动态分化。
It has been demonstrated that through the formation of embryoid bodies (EBs) germ cells can be derived from embryonic stem (ES) cells. Here, we describe a transgene expression approach to derive germ cells directly from ES cells in vitro without EB formation. Through the ectopic expression of Deleted in Azoospermia-Like (Dazl), a germ cell-specific RNA-binding protein, both motile tailed-sperm and oocytes were induced from mouse ES (mES) cells in culture. Furthermore, transient overexpression of Dazl led to suppression of Nanog but induced germ cell nuclear antigen in mES cells. Dazl knockdown resulted in reduction in the expression of germ cell markers including Stella, MVH and Prdm1. Our study indicates that Dazl is a master gene controlling germ cell differentiation and that ectopic expression of Dazl promotes the dynamic differentiation of mouse ES cells into gametes in vitro.