Assay miniaturization for ultra-high throughput screening of combinatorial and discrete compound libraries: A 9600-well (0.2 microliter) assay system
Assay miniaturization for ultra-high throughput screening of combinatorial and discrete compound libraries: A 9600-well (0.2 microliter) assay system
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DOI：
10.1177/108705719800300108
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发表时间：
1998-03-01
影响因子：
--
通讯作者：
Chung, TDY
中科院分区：
文献类型：
--
作者：
Oldenburg, KR;Zhang, JH;Chung, TDY
组合化学在寻找新的治疗方法方面开辟了化学实体的新领域，组合化学目前正在增加成千上万的化合物到类似的数量；可以预见，在未来几年，将有近100万种化合物可用于针对每种治疗靶点进行筛选。随着基因组学的进步，潜在靶点的数量也将增加，随着越来越多的化合物需要针对越来越多的靶点进行筛选。获得筛选这些化合物所需的关键生物材料的难度和成本越来越高，一个显而易见的解决方案是使分析方法小型化，这样就不需要增加生物试剂的供应。为此，我们开发了一种超高通量筛选系统，该系统由新的板设计（9600孔）、检测系统和液体处理系统组成。这种新格式能够以0.2 μ l的速度进行分析。与标准的96孔格式相比，该系统获得的结果更优。
Combinatorial chemistry has opened a new realm of chemical entities in the search for novel therapeutics, Combinatorial chemistry is currently adding hundreds of thousands of compounds to similar numbers available from yea;rs of synthesis by medicinal chemistry, It is not unreasonable to expect that over the next several years, nearly a million compounds will be available for screening against each therapeutic target, The number of potential targets will also be increasing with the advances in genomics, With the increasing number of compounds to be screened against an increasing number of targets, it is becoming increasingly difficult and costly to obtain the required amounts of key biological material needed to screen these compounds, One obvious solution is to miniaturize the assays so that the biological reagent supply doesn't need to increase, To this end, we have developed an ultra high throughput screening system comprised of a new plate design (9600-well), detection system, and liquid handling system, This new format is capable of performing assays in as little as 0.2 mu l. The results obtained from this system compare favorably to those obtained in the standard 96-well format.